New technique for needle-less implantation of eukaryotic cells

Cytotherapy
Luis Fernando Arenas da SilvaArnulf Stenzl

Abstract

On review of the use of stem cells in the literature, promissory outcomes for functional organ recovery in many subspecialties in medicine underscore its therapeutic potential. The application of stem cells through the use of a needle can result in additional scar formation, which is undesired for delicate organs. The present work describes the use of a needle-less stem cell injector with the Immediate Drop on Demand Technology (I-DOT) for cell injection in vitro. Mesenchymal stromal cells from human bone marrow were labeled with ethynyl-deoxyuridine (EdU) for 2 days and then were re-suspended. With the use of I-DOT, the cells were applied to type 1 collagen matrices or pig bladder tissue specimens with or without mucosa at different levels of energy. The collagen matrices were analyzed after 4 h and 5 days; bladder tissue specimens were analyzed 4 h after cell implantation. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test (MTT) assay was performed immediately after cell application to the collagen matrices. Histological analysis with the use of frozen sections and immunofluorescence was used to localize EdU-labeled cells. A considerable number of cells were detected by use of the MTT assay for collagen matri...Continue Reading

References

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Dec 20, 2014·Journal of Laboratory Automation·Lena SchoberThomas Bauernhansl

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Citations

Mar 17, 2020·Computers in Biology and Medicine·Abdul Mohizin, Jung Kyung Kim

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