New tools for chloroplast genetic engineering allow the synthesis of human growth hormone in the green alga Chlamydomonas reinhardtii

Applied Microbiology and Biotechnology
Thanyanan WannathongSaul Purton

Abstract

In recent years, there has been an increasing interest in the exploitation of microalgae in industrial biotechnology. Potentially, these phototrophic eukaryotes could be used for the low-cost synthesis of valuable recombinant products such as bioactive metabolites and therapeutic proteins. The algal chloroplast in particular represents an attractive target for such genetic engineering, both because it houses major metabolic pathways and because foreign genes can be targeted to specific loci within the chloroplast genome, resulting in high-level, stable expression. However, routine methods for chloroplast genetic engineering are currently available only for one species-Chlamydomonas reinhardtii-and even here, there are limitations to the existing technology, including the need for an expensive biolistic device for DNA delivery, the lack of robust expression vectors, and the undesirable use of antibiotic resistance markers. Here, we describe a new strain and vectors for targeted insertion of transgenes into a neutral chloroplast locus that (i) allow scar-less fusion of a transgenic coding sequence to the promoter/5'UTR element of the highly expressed endogenous genes psaA or atpA, (ii) employ the endogenous gene psbH as an effect...Continue Reading

References

Nov 1, 1986·Antimicrobial Agents and Chemotherapy·M Arthur, P Courvalin
Nov 1, 1980·The Journal of Clinical Endocrinology and Metabolism·T TanakaH G Friesen
Nov 1, 1996·Biulleten' eksperimental'noĭ biologii i meditsiny·A A BulatovG V Raevskaia
Mar 4, 2000·Nature Biotechnology·J M StaubD A Russell
Aug 12, 2000·Journal of Experimental Botany·K Maxwell, G N Johnson
Mar 7, 2001·Antioxidants & Redox Signaling·K W LauM Wu
Oct 12, 2001·The Plant Cell·D EspositoD B Stern
Mar 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·K L KindleD B Stern
Oct 2, 2002·FEBS Letters·Yves Choquet, Francis-André Wollman
Jan 11, 2003·Proceedings of the National Academy of Sciences of the United States of America·Stephen P MayfieldRichard A Lerner
Oct 20, 2005·Journal of Bioscience and Bioengineering·Seitaro KasaiAstuhiko Shinmyo
Mar 16, 2007·Plant Biotechnology Journal·Andrea L ManuellStephen P Mayfield
Dec 29, 2007·Advances in Experimental Medicine and Biology·Saul Purton
Jun 30, 2009·Biotechnology and Bioengineering·Miller TranStephen P Mayfield
Dec 10, 2009·Journal of Biotechnology·Imke A J DreesenMartin Fussenegger
Sep 3, 2010·Plant Biotechnology Journal·Laure MicheletMichel Goldschmidt-Clermont
Mar 24, 2011·Plant Biotechnology Journal·Anil Day, Michel Goldschmidt-Clermont
May 5, 2011·Archives of Disease in Childhood·Jeremy Kirk
Sep 2, 2011·Journal of Industrial Microbiology & Biotechnology·Yangmin GongHong Gao
Feb 2, 2012·Organic & Biomolecular Chemistry·Cormac D Murphy
Nov 22, 2012·Protein Expression and Purification·Jonathan T Sockolosky, Francis C Szoka
Dec 14, 2012·Proceedings of the National Academy of Sciences of the United States of America·Miller TranStephen P Mayfield
Apr 23, 2013·Applied and Environmental Microbiology·James A GregoryStephen Mayfield
May 8, 2013·Plant Biotechnology Journal·Hsu-Ching Chen, Anastasios Melis
May 8, 2013·Current Opinion in Biotechnology·René H WijffelsKlaas J Hellingwerf
May 10, 2013·ACS Synthetic Biology·Elizabeth A Specht, Stephen P Mayfield
May 31, 2013·Biotechnology and Bioengineering·Miller TranStephen P Mayfield
Mar 7, 2014·Methods in Molecular Biology·Chloe EconomouSaul Purton
Aug 15, 2014·Biotechnology and Bioengineering·Javier A GimpelStephen P Mayfield
Sep 18, 2014·Plant Biotechnology Journal·Daniel J BarreraStephen P Mayfield
Feb 3, 2015·The Plant Journal : for Cell and Molecular Biology·Mark A ScaifeAlison G Smith

❮ Previous
Next ❯

Citations

Nov 9, 2016·Applied Microbiology and Biotechnology·Janeth I GalarzaVitalia Henríquez
Apr 13, 2018·Biology·Patai CharoonnartVanvimon Saksmerprome
Sep 21, 2019·Biotechnology and Applied Biochemistry·Ayesha SiddiquiNiaz Ahmad
Mar 7, 2020·Expert Opinion on Biological Therapy·Sergio Rosales-MendozaBernardo Bañuelos-Hernandez
Nov 28, 2018·World Journal of Microbiology & Biotechnology·Yong Min KwonJaoon Young Hwan Kim
Nov 12, 2018·Microbial Cell Factories·Manuel BenedettiLuca Dall'Osto
Dec 24, 2019·Frontiers in Bioengineering and Biotechnology·Laura StoffelsBrenda Parker
Dec 22, 2019·Biotechnology Advances·Jaber DehghaniYadollah Omidi
Apr 4, 2021·Biology·Sergio Gutiérrez, Kyle J Lauersen
Aug 13, 2021·Frontiers in Plant Science·Gregory M NewkirkJuan Pablo Giraldo
Aug 28, 2021·International Journal of Molecular Sciences·Marco Larrea-Álvarez, Saul Purton
Aug 21, 2020·Trends in Biotechnology·Shih-Hsin HoWei-Hsin Chen

❮ Previous
Next ❯

Datasets Mentioned

BETA
CC-5168
KU179444

Methods Mentioned

BETA
glycosylations
transgenics
transgenic
PCR
Infrared Imaging

Software Mentioned

softberry BPROM
same

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.