New tools for detecting latent tuberculosis infection: evaluation of RD1-specific long-term response.

BMC Infectious Diseases
O ButeraDelia Goletti

Abstract

Interferon-gamma (IFN-gamma) release assays (IGRAs) were designed to detect latent tuberculosis infection (LTBI). However, discrepancies were found between the tuberculin skin test (TST) and IGRAs results that cannot be attributed to prior Bacille Calmètte Guerin vaccinations. The aim of this study was to evaluate tools for improving LTBI diagnosis by analyzing the IFN-gamma response to RD1 proteins in prolonged (long-term response) whole blood tests in those subjects resulting negative to assays such as QuantiFERON-TB Gold In tube (QFT-IT). The study population included 106 healthy TST+ individuals with suspected LTBI (recent contact of smear-positive TB and homeless) consecutively enrolled. As controls, 13 healthy subjects unexposed to M. tuberculosis (TST-, QFT-IT-) and 29 subjects with cured pulmonary TB were enrolled. IFN-gamma whole blood response to RD1 proteins and QFT-IT were evaluated at day 1 post-culture. A prolonged test evaluating long-term IFN-gamma response (7-day) to RD1 proteins in diluted whole blood was performed. Among the enrolled TST+ subjects with suspected LTBI, 70/106 (66.0%) responded to QFT-IT and 64/106 (60.3%) to RD1 proteins at day 1. To evaluate whether a prolonged test could improve the detectio...Continue Reading

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Citations

Feb 28, 2014·Immunobiology·Om Prakash Singh, Shyam Sundar
Oct 30, 2012·The Journal of Infection·Filippo BartalesiAlessandro Bartoloni
May 18, 2010·The Journal of Infection·Delia GolettiPhilippe Henri Lagrange
Aug 9, 2015·The Journal of Infection·Elisa PetruccioliDelia Goletti
Mar 7, 2013·The Journal of Infection·Elisa PetruccioliDelia Goletti
Jan 25, 2017·BMC Research Notes·Leonardo Silva de AraujoMaria Helena Féres Saad
Jun 1, 2018·Expert Review of Anti-infective Therapy·Delia GolettiFabrizio Cantini
Mar 1, 2012·Expert Review of Molecular Diagnostics·Morten RuhwaldPernille Ravn
Apr 4, 2012·Expert Review of Molecular Diagnostics·Delia Goletti, Martina Sester
Jun 17, 2016·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Violette DirixUNKNOWN TB-IRIS study group
Dec 31, 2019·Journal of Immunology Research·Eleane de OyarzabalMartha Torres

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISA
Enzyme Linked ImmunoSorbent Assay

Software Mentioned

LABROC
Prism
SPSS

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