Abstract
Chicken peripheral blood lymphocytes (PBL) were infected with the GB strain of Newcastle disease virus (NDV). At 0, 1.5 and 3 h postinfection, PBL were stained with acridine orange-ethidium bromide and examined for apoptotic and necrotic indices under a UV microscope. At the end of 3 h, the cells were pelleted and were either fixed for electron microscopy or used for DNA extraction. Infected cells showed a significant amount of apoptosis and necrosis when compared with controls. Electron microscopy of the infected cells also showed characteristics of apoptosis, i.e. apoptotic bodies, margination of chromatin with crescent formation and fusion of cells. DNA extracted from virus-infected cells showed extensive fragmentation. Based on these findings it is concluded that NDV, in addition to causing necrosis in chicken lymphocytes, can induce apoptosis.
References
Dec 1, 1992·Journal of Virology·S H JeurissenM H Noteborn
Jul 10, 1992·Science·L MeyaardF Miedema
Apr 1, 1991·Immunology Today·J C Ameisen, A Capron
Dec 1, 1991·Virology·A G Laurent-CrawfordA G Hovanessian
Apr 19, 1991·Science·A W HüginH C Morse
Aug 1, 1991·European Journal of Immunology·B Motyka, J D Reynolds
Oct 1, 1987·Veterinary Microbiology·K M Lam, Q Hao
Oct 1, 1972·Cellular Immunology·J F Woodruff, J J Woodruff
Sep 1, 1970·Cellular Immunology·J F Woodruff, J J Woodruff
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·R C DukeJ J Cohen
Jan 1, 1984·The Journal of Pathology·A H WyllieD Dunlop
Jan 1, 1980·International Review of Cytology·A H WyllieA R Currie
Mar 1, 1993·Cellular Immunology·J AsakawaG J Thorbecke
Citations
Feb 22, 2008·Archives of Virology·P V RavindraR S Chauhan
Dec 1, 1995·Veterinary Microbiology·K M Lam
Oct 1, 1996·Journal of Comparative Pathology·K M Lam
Mar 16, 2000·Developmental and Comparative Immunology·B S SealH S Sellers
Oct 1, 1996·Veterinary Immunology and Immunopathology·K M LamJ P Eiserich
Jul 1, 1997·Veterinary Immunology and Immunopathology·K M Lam
Feb 19, 2000·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·J G Sinkovics, J C Horvath
Jun 15, 2010·Avian Pathology : Journal of the W.V.P.A·Daoliang LanHua Yue
Jul 8, 2003·American Journal of Therapeutics·József SzeberényiLaszlo K Csatary
Jan 12, 2007·Journal of Virology·Zsolt FábiánLaszlo K Csatary
Mar 4, 2010·Genetics and Molecular Research : GMR·H L Del PuertoA C Vasconcelos
Jan 11, 2000·Journal of Comparative Pathology·K M Lam, A J DaMassa
Dec 26, 2001·Veterinary Microbiology·Chengbin LinThomas M Chambers
Nov 26, 2015·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Yaling LiTao Ren
Jul 30, 2015·Virus Research·Sara Cuadrado-CastanoEnrique Villar
May 1, 1995·Journal of Comparative Pathology·A C Vasconcelos, K M Lam
Jan 1, 1996·Journal of Comparative Pathology·K M Lam
Nov 1, 1996·Journal of Comparative Pathology·L CarrascoM A Sierra
May 17, 2002·Veterinary Pathology·G D KommersC C Brown
Apr 26, 2003·Avian Diseases·K M Lam
Jul 31, 2003·Avian Diseases·Glaucia D KommersCorrie C Brown
Jan 23, 2013·International Journal of Molecular Medicine·Mohamed GhriciAini Ideris
Sep 12, 2006·The Journal of General Virology·Senthilkumar NatesanA Sylvester
Dec 1, 1996·Journal of Virology·B L HaagmansM C Horzinek