Newcastle disease virus (NDV) expressing the spike protein of SARS-CoV-2 as vaccine candidate

BioRxiv : the Preprint Server for Biology
Weina SunPeter Palese

Abstract

Due to the lack of protective immunity of humans towards the newly emerged SARS-CoV-2, this virus has caused a massive pandemic across the world resulting in hundreds of thousands of deaths. Thus, a vaccine is urgently needed to contain the spread of the virus. Here, we describe Newcastle disease virus (NDV) vector vaccines expressing the spike protein of SARS-CoV-2 in its wild type or a pre-fusion membrane anchored format. All described NDV vector vaccines grow to high titers in embryonated chicken eggs. In a proof of principle mouse study, we report that the NDV vector vaccines elicit high levels of antibodies that are neutralizing when the vaccine is given intramuscularly. Importantly, these COVID-19 vaccine candidates protect mice from a mouse-adapted SARS-CoV-2 challenge with no detectable viral titer and viral antigen in the lungs.

Datasets Mentioned

BETA
MN908947.3
of

Methods Mentioned

BETA
PCR
transfection
electrophoresis
ELISAs

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