Newcastle Disease Virus (NDV) Oncolytic Activity in Human Glioma Tumors Is Dependent on CDKN2A-Type I IFN Gene Cluster Codeletion.

Cells
Noemi García-RomeroAngel Ayuso-Sacido

Abstract

Glioblastoma (GBM) is the most aggressive and frequent primary brain tumor in adults with a median overall survival of 15 months. Tumor recurrence and poor prognosis are related to cancer stem cells (CSCs), which drive resistance to therapies. A common characteristic in GBM is CDKN2A gene loss, located close to the cluster of type I IFN genes at Ch9p21. Newcastle disease virus (NDV) is an avian paramyxovirus with oncolytic and immunostimulatory properties that has been proposed for the treatment of GBM. We have analyzed the CDKN2A-IFN I gene cluster in 1018 glioma tumors and evaluated the NDV oncolytic effect in six GBM CSCs ex vivo and in a mouse model. Our results indicate that more than 50% of GBM patients have some IFN deletion. Moreover, GBM susceptibility to NDV is dependent on the loss of the type I IFN. Infection of GBM with an NDV-expressing influenza virus NS1 protein can overcome the resistance to oncolysis by NDV of type I-competent cells. These results highlight the potential of using NDV vectors in antitumor therapies.

References

May 1, 1992·The Journal of Surgical Research·K W ReichardJ A Greager
Oct 1, 1993·Hybridoma·V MöbusV Schirrmacher
Mar 4, 1998·Head & Neck·W M LydiattR S Chaganti
Jan 5, 2002·Journal of Neuropathology and Experimental Neurology·T WatanabeH Ohgaki
Aug 14, 2003·Journal of Virology·Man-Seong ParkPeter Palese
Sep 29, 2004·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Hans Herbert SteinerChristel Herold-Mende
Mar 11, 2005·The New England Journal of Medicine·Roger StuppUNKNOWN National Cancer Institute of Canada Clinical Trials Group
Nov 1, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Arnold I FreemanTali Siegal
Feb 14, 2006·International Journal of Cancer. Journal International Du Cancer·Christoph FiolaVolker Schirrmacher
May 24, 2006·Proceedings of the National Academy of Sciences of the United States of America·Man-Seong ParkPeter Palese
Sep 15, 2006·Journal of Virology·Zejun LiHualan Chen
Sep 7, 2007·Cancer Research·Adam VigilAdolfo García-Sastre
Feb 12, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Dmitriy ZamarinYuman Fong
May 7, 2010·CA: a Cancer Journal for Clinicians·Erwin G Van MeirJeffrey J Olson
Mar 8, 2012·Future Microbiology·Dmitriy Zamarin, Peter Palese
Dec 12, 2012·Molecular and Cellular Biology·Amir ApelbaumGideon Schreiber
Apr 19, 2013·Cell Transplantation·Der-Yang ChoLi-Hui Ho
Oct 23, 2013·Journal of Visualized Experiments : JoVE·Juan AyllonLuis Martínez-Sobrido
Dec 24, 2013·Nature Reviews. Immunology·Lionel B Ivashkiv, Laura T Donlin
Mar 5, 2014·Virus Research·Ketan GanarSachin Kumar
Jul 24, 2014·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Jigisha P ThakkarJohn L Villano
Sep 12, 2014·International Journal of Cancer. Journal International Du Cancer·Carolien A KoksStefaan W Van Gool
Jan 8, 2015·Frontiers in Cellular Neuroscience·Luiz Gustavo DuboisVivaldo Moura-Neto
Feb 2, 2015·Current Opinion in Virology·Robert M Krug
Apr 9, 2015·Journal of Neuropathology and Experimental Neurology·Gerald F ReisArie Perry
May 23, 2015·Human Vaccines & Immunotherapeutics·Pascal R A BuijsBernadette G van den Hoogen
Jun 2, 2015·Nature Reviews. Immunology·Laurence ZitvogelGuido Kroemer
Aug 8, 2015·Nature Medicine·Ash A AlizadehJessica Zucman-Rossi
Feb 18, 2016·Chinese Medical Journal·Qiu-Ju LiCheng-Yin Liu
Feb 18, 2016·Stem Cells International·Brandon D LiebeltJian Hu
Feb 26, 2016·Nature Reviews. Cancer·Belinda S ParkerPaul J Hertzog
Aug 2, 2016·Journal of the Egyptian National Cancer Institute·Supriya MallickGoura K Rath
Feb 13, 2018·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Frederick F LangJuan Fueyo

❮ Previous
Next ❯

Citations

Oct 18, 2020·International Journal of Molecular Sciences·Sergio Rius-RocabertEstanislao Nistal-Villan
Mar 28, 2021·Annals of Oncology : Official Journal of the European Society for Medical Oncology·A B CarpenterS Hanft
Mar 31, 2021·Neurosurgery Clinics of North America·Dagoberto Estevez-OrdonezJames M Markert

❮ Previous
Next ❯

Methods Mentioned

BETA
genetic modification
transfect
PCRs
PCR
Assay

Software Mentioned

Image J
CellProfiler
GraphPad Prism

Related Concepts

Related Feeds

Cancer Stem Cells in Glioblastoma

Glioblastoma is the most common and aggressive type of brain tumor. It contains a population of tumor initiating stem cell-like cells known as cancer stem cells. Investigations are ongoing into these cancer stem cells found in these solid tumors which are highly resistance to treatment. Here is the latest research on cancer stem cells in glioblastoma.