Abstract
We have recently developed a primary culture system for visceral adipocytes (VAs) using stomal-vascular cells (SVCs) isolated from the mesenteric fat tissue of male Sprague-Dawley rats of 3-5 weeks of age. Modified Dulbecco's modified Eagle medium (DMEM)/F12 containing 17 microM pantothenic acid, 33 microM biotin, 100 microM ascorbic acid, 1 microM octanoic acid, 50 nM triiodothyronine, 10 microg/ml insulin, 10% newborn calf serum (NCS), 100 units/ml penicillin and 100 microg/ml streptomycin was used as a basal culture medium, which did not contain any synthetic compounds usually used to promote adipogenesis, such as indomethacin, dexamethasone, or peroxisome proliferator-activated receptor (PPAR)-gamma agonists. The SVCs differentiated and proliferated efficiently, and formed a confluent monolayer in 3 days. The VAs accumulated lipids droplets in their cytoplasm at approximately 7 days. The differentiation rate from applied SVCs to mature adipocytes was >80% per culture. Adiponectin concentration in the medium increased from Day 5 to Day 7. Application of lipid emulsion stimulated maturation of the SVCs into VAs, as well as subsequent lipid accumulation. Norepinephrine (2 x 10(-5) mM) reduced the size of lipid particles and de...Continue Reading
Citations
Sep 22, 2010·The British Journal of Nutrition·Tomoji OnoHoyoku Nishino
Jun 9, 2011·Journal of Gastroenterology and Hepatology·Norihito KuboAkio Nakane
Apr 21, 2010·Pathology International·Kazuyoshi UchihashiShuji Toda
Feb 16, 2011·Diabetes, Obesity & Metabolism·A K StunesB I Gustafsson
Jul 10, 2010·Journal of Biomaterials Science. Polymer Edition·Takashige SatoJin-ichi Inokuchi
Oct 20, 2012·Journal of Lipid Research·Sayaka Akieda-AsaiYukari Date
Nov 21, 2008·Journal of Lipid Research·Takeshi HashimotoHiroaki Kosaka
Mar 9, 2013·Bioscience, Biotechnology, and Biochemistry·Tomoji OnoHoyoku Nishino
Nov 10, 2009·FEBS Letters·Masafumi NagayamaKazutoshi Gohara
Mar 12, 2015·Journal of Clinical Biochemistry and Nutrition·Risa TairaToshio Taira
Jun 30, 2006·Journal of Neurochemistry·Katsuhiko Mikoshiba
Nov 26, 2010·Biochemical and Biophysical Research Communications·Atsushi MomoseJunji Yamada
Apr 22, 2014·Molecular Metabolism·Denise S Fernandez-TwinnSusan E Ozanne
Oct 12, 2014·Glycobiology·Masakazu NagafukuJin-Ichi Inokuchi
Jan 27, 2017·The Journal of Veterinary Medical Science·Jun A MinaguchiKazushige Takehana
Oct 24, 2006·Journal of Lipid Research·Masafumi NagayamaKazutoshi Gohara
Sep 10, 2008·Cell Biology International·Takashige SatoJin-ichi Inokuchi
Sep 3, 2016·Journal of Veterinary Science·Masahiro YasudaYukari Date