Newly identified bacteriophages carrying the stx2g Shiga toxin gene isolated from Escherichia coli strains in polluted waters

FEMS Microbiology Letters
Cristina García-AljaroAnicet R Blanch

Abstract

Shiga toxin-converting bacteriophages are the main vehicle involved in horizontal transmission of stx1 and stx2 genes, which has led to the current spread of stx genes among a high number of Escherichia coli serotypes and other enterobacteria. Several stx gene variants have been described, although some of the variants have never been isolated from inducible bacteriophages. In this study, two stx2g-carrying bacteriophages induced from two different E. coli O2:H25 strains isolated from different wastewater samples were characterized. These bacteriophages when transduced into Shigella sonnei retained their ability to produce Stx2 protein. They had similar but not identical DNA restriction patterns, and similar host ranges. Electron microscopy studies showed that they had isometric capsids with short tails, which resembled phage 933W. However, DNA cross-hybridization studies showed that phage 933W was not closely related to stx2g bacteriophages. This first description of stx2g-carrying bacteriophages enlarges the list of stx-carrying bacteriophages involved in horizontal transmission of stx genes in the environment.

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Citations

Mar 17, 2007·Biotechnology Letters·Mikael SkurnikSaija Kiljunen
Jul 14, 2010·Applied and Environmental Microbiology·Lejla ImamovicMaite Muniesa
Nov 18, 2008·Applied and Environmental Microbiology·Cristina García-AljaroAnicet R Blanch
Nov 30, 2011·Future Microbiology·Veronica Casas, Stanley Maloy
Dec 3, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Domonkos SvábIstván Tóth
May 2, 2014·Journal of Food Protection·Peter C H Feng, Shanker P Reddy
Jan 5, 2021·Canadian Journal of Microbiology·Juan Martín Talavera-GonzálezCelene Salgado-Miranda

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