Newly-synthesized beta-tubulin demonstrates domain-specific interactions with the cytosolic chaperonin

Biochemistry
J K DobrzynskiH Sternlicht

Abstract

Tubulin folding requires two chaperone systems, i.e., the 900 kDa cytosolic chaperonin referred to as the TCP-1 complex or TRiC which facilitates folding of the alpha- and beta-tubulin subunits and a ca. 180 kDa complex which facilitates further assembly into heterodimer. beta-Tubulin mutants were expressed in rabbit reticulocyte lysates, and the effect of C-terminal, N-terminal, and internal deletions on the binding of beta-tubulin polypeptides to the 900 and 180 kDa complexes was ascertained. Proteolytic studies of chaperonin-bound beta-tubulin were also implemented. These studies support the concept of quasi-native chaperonin-bound intermediates [Tian et al. J. Biol. Chem. (1995) 270, 1-4]. Three "domains" similar in size to the domains in the native protein were implicated in facilitated folding: i.e., an internal or "M-domain" composed of residues approximately 140-260 which binds to TRiC; a "C-domain" composed of residues approximately 300-445 which interacts less strongly with TRiC and may contain regulatory sequences for tubulin release from the chaperonin; and an "N-domain" composed of residues approximately 1-140 which apparently does not interact with TRiC but does interact with the 180 kDa complex. The major TRiC-in...Continue Reading

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Citations

Dec 11, 2002·European Journal of Biochemistry·Sandra PucciarelliRonald Melki
Jun 8, 2001·Annual Review of Biochemistry·J Frydman
Feb 7, 2001·Current Biology : CB·M R Leroux, F U Hartl
Apr 9, 2008·Archives of Biochemistry and Biophysics·Maria Giulia Bigotti, Anthony R Clarke
Oct 3, 2001·Journal of Structural Biology·A Y DunnJ Frydman
Feb 9, 2012·Journal of Amino Acids·M Anaul KabirZulfiqar Ahmad
Nov 10, 2000·Journal of Molecular Biology·M Ritco-Vonsovici, K R Willison
Jan 16, 1999·The Journal of Biological Chemistry·A RoobolM J Carden
Sep 6, 2001·The Journal of Biological Chemistry·H RommelaereC Ampe
Sep 16, 1998·Antimicrobial Agents and Chemotherapy·D W HollomonL Hall

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