PMID: 7009791Mar 1, 1981Paper

Newly synthesized cholecystokinin in subcellular fractions of the rat brain

Journal of Neurochemistry
N R GoltermannN J Christensen

Abstract

The subcellular localization of in vivo synthesized cholecystokinin (CCK) in different parts of the rat brain was studied after intracisternal pulse injections of [35S]methionine. The rats were decapitated 1 h after the injection, and the brain was divided into cortex, hippocampus and remainder. Subcellular fractions were obtained according to Whittaker's method. De novo synthesized CCK in the crude mitochondrial-synaptosomal fraction, P2, and in the purified synaptosomal fraction was demonstrated by affinity chromatography, using antibodies specific for the COOH-terminal sequence of CCK. By subsequent gel chromatography two molecular forms of labelled CCK occurred, with elution constants, Kav, of 1.1 (corresponding to the COOH-terminal octapeptide) and of 1.40 (a component which may correspond to the COOH-terminal tetrapeptide amide, CCK-4). The findings support the idea that the small molecular forms are the transmitter forms of CCK.

References

Dec 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M PingetR S Yalow
Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R B InnisS H Snyder
Apr 1, 1979·Journal of Neurochemistry·J F Rehfeld, N R Goltermann
Aug 1, 1980·Journal of Neurochemistry·N R GoltermannH R Petersen

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Citations

Mar 1, 1986·Neuroscience and Behavioral Physiology·E E VasarL Kh Allikmets
Jan 1, 1994·Neuroscience and Biobehavioral Reviews·F J Vaccarino
Apr 12, 2003·Pharmacology & Toxicology·Susan RotzingerFranco J Vaccarino
Sep 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·K Stengaard-PedersenJ F Rehfeld
Oct 1, 1983·Neuropeptides·M C Beinfeld
Jan 1, 1985·Annals of the New York Academy of Sciences·N R Goltermann
Oct 1, 1986·Neuropeptides·T Moroji, Y Hagino
Oct 15, 1993·The Journal of Comparative Neurology·K M Oeth, D A Lewis
Jan 15, 1989·The Journal of Comparative Neurology·K B Seroogy, J H Fallon
Nov 1, 1990·The Journal of Comparative Neurology·K M Oeth, D A Lewis

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