Next-generation Raman tomography instrument for non-invasive in vivo bone imaging

Biomedical Optics Express
Jennifer-Lynn H DemersBrian W Pogue

Abstract

Combining diffuse optical tomography methods with Raman spectroscopy of tissue provides the ability for in vivo measurements of chemical and molecular characteristics, which have the potential for being useful in diagnostic imaging. In this study a system for Raman tomography was developed and tested. A third generation microCT coupled system was developed to combine 10 detection fibers and 5 excitation fibers with laser line filtering and a Cytop reference signal. Phantom measurements of hydroxyapatite concentrations from 50 to 300 mg/ml had a linear response. Fiber placement and experiment design was optimized using cadaver animals with live animal measurements acquired to validate the systems capabilities. Promising results from the initial animal experiments presented here, pave the way for a study of longitudinal measurements during fracture healing and the scaling of the Raman tomography system towards human measurements.

References

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Citations

Dec 15, 2015·Analytical Chemistry·Thomas Wilhelm BocklitzJuergen Popp
Oct 28, 2015·Biomedical Optics Express·Asael PapourWarren Grundfest
Aug 6, 2016·Analytical and Bioanalytical Chemistry·Karen A Esmonde-WhiteIan R Lewis
Sep 9, 2016·Optics Express·Fabrizio MartelliAntonio Pifferi
Apr 25, 2017·Nature Communications·Xueli ChenJi-Xin Cheng
Mar 27, 2018·Journal of Biophotonics·Mustafa UnalJeffry S Nyman
Apr 11, 2019·Journal of Biomedical Optics·Paul Kumar Upputuri, Manojit Pramanik
Oct 24, 2018·Journal of Biomedical Optics·Brian W Pogue, Brian C Wilson
Nov 19, 2019·Frontiers in Bioengineering and Biotechnology·Mads S BergholtMichael B Albro
Sep 5, 2020·Biomedical Engineering and Computational Biology·Martha Z Vardaki, Nikolaos Kourkoumelis
Jun 22, 2018·Materials·Abdennasser ChekrounJean-Philippe Berteau
Nov 11, 2020·Chemical Society Reviews·Fay NicolsonPavel Matousek
Feb 13, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·William QueridoNancy Pleshko
Nov 18, 2021·The Analyst·Mustafa UnalJeffry S Nyman

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