Next-generation sequencing-based transcriptome analysis of Cryptolaemus montrouzieri under insecticide stress reveals resistance-relevant genes in ladybirds

Genomics
Yuhong ZhangHong Pang

Abstract

As the most efficient natural enemy of mealybugs, the ladybird Cryptolaemus montrouzieri Mulsant plays an important role in integrated pest management. We report here a profiling analysis of C. montrouzieri under insecticide stress to gain a deeper view of insecticide resistance in ladybirds. For transcriptome sequencing, more than 26 million sequencing reads were produced. These reads were assembled into 38,369 non-redundant transcripts (mean size=453 nt). 23,248 transcripts were annotated with their gene description. Using a tag-based DGE (Digital gene expression) system, over 5.7 million tags were sequenced in both the insecticide stress group and the control group, and mapped to 38,369 transcripts. We obtained 993 genes that were significantly up- or down-regulated under insecticide stress in the ladybird transcriptome. These results can contribute to in-depth research into the molecular mechanisms of resistance and enhance our current understanding of the effects of insecticides on natural enemies.

References

Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·M B EisenD Botstein
Dec 11, 1999·Nucleic Acids Research·R L TatusovE V Koonin
Dec 19, 2003·Nucleic Acids Research·Minoru KanehisaMasahiro Hattori
Jun 8, 2004·Bioinformatics·Alok J Saldanha
Apr 12, 2005·Annual Review of Pharmacology and Toxicology·Motohiro Tomizawa, John E Casida
Sep 21, 2005·Invertebrate Neuroscience : in·Valérie Raymond-DelpechDavid B Sattelle
Jul 18, 2006·Nucleic Acids Research·Jia YeJun Wang
Jul 18, 2006·Nucleic Acids Research·Jian YeThomas L Madden
Mar 20, 2008·Genome Research·Daniel R Zerbino, Ewan Birney
May 3, 2008·Science·Ugrappa NagalakshmiMichael Snyder
Jun 3, 2008·Nature Methods·Ali MortazaviBarbara Wold
Jun 14, 2008·Genome Research·Weichun Huang, Gabor Marth
Aug 16, 2008·Genomics·Olena Morozova, Marco A Marra
Oct 3, 2008·Genome Biology·Nicole Cloonan, Sean M Grimmond
Nov 19, 2008·Nature Reviews. Genetics·Zhong WangMichael Snyder
Apr 25, 2009·PLoS Genetics·John S Mattick
Jun 23, 2009·Genome Research·A Sorana MorrissyMarco A Marra
Dec 31, 2010·Nature Reviews. Genetics·Fatih Ozsolak, Patrice M Milos
Jan 11, 2011·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Rohini GargMukesh Jain

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Citations

Aug 14, 2015·Scientific Reports·Jiaqin XieHong Pang
Apr 16, 2019·Frontiers in Physiology·Zbigniew AdamskiGrzegorz Rosiński
Mar 29, 2019·Environmental Science & Technology·Ning HuangXiaoyu Wang

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