Next-generation sequencing detects repetitive elements expansion in giant genomes of annual killifish genus Austrolebias (Cyprinodontiformes, Rivulidae)

Genetica
G GarcíaV Gutiérrez

Abstract

Among Neotropical fish fauna, the South American killifish genus Austrolebias (Cyprinodontiformes: Rivulidae) constitutes an excellent model to study the genomic evolutionary processes underlying speciation events. Recently, unusually large genome size has been described in 16 species of this genus, with an average DNA content of about 5.95 ± 0.45 pg per diploid cell (mean C-value of about 2.98 pg). In the present paper we explore the possible origin of this unparallel genomic increase by means of comparative analysis of the repetitive components using NGS (454-Roche) technology in the lowest and highest Rivulidae genomes. Here, we provide the first annotated Rivulidae-repeated sequences composition and their relative repetitive fraction in both genomes. Remarkably, the genomic proportion of the moderately repetitive DNA in Austrolebias charrua genome represents approximately twice (45%) of the repetitive components of the highly related rivulinae taxon Cynopoecilus melanotaenia (25%). Present work provides evidence about the impact of the repeat families that could be distinctly proliferated among sublineages within Rivulidae fish group, explaining the great genome size differences encompassing the differentiation and speciati...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Oct 6, 1999·Genome Research·X Huang, A Madan
Oct 11, 2001·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·G GarcíaM Cappetta
Jul 27, 2002·Science·Samuel AparicioSydney Brenner
Dec 3, 2003·Trends in Genetics : TIG·Jean-Nicolas VolffCécile Fischer
Jan 28, 2005·Heredity·J-N Volff
Jan 28, 2010·Gene·Rita RebolloCristina Vieira
Oct 22, 2011·Mobile Genetic Elements·Daniela Cristina FerreiraFausto Foresti
Jul 24, 2013·Genome Research·Masato NikaidoNorihiro Okada
Dec 3, 2013·Briefings in Functional Genomics·Herman P SpainkRon P Dirks
Nov 1, 1998·Journal of Morphology·Marcelo Loureiro, Rafael O De Sá

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Citations

Jun 14, 2017·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Nibia BeroisRafael Omar De Sá
Sep 1, 2017·Frontiers in Genetics·Ricardo UtsunomiaFausto Foresti
Jul 14, 2018·Mobile DNA·Alexandre Freitas da SilvaGabriel Luz Wallau

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