Next generation sequencing from Hepatozoon canis (Apicomplexa: Coccidia: Adeleorina): Complete apicoplast genome and multiple mitochondrion-associated sequences

International Journal for Parasitology
Alexandre N LeveilleJohn R Barta

Abstract

Extrachromosomal genomes of the adeleorinid parasite Hepatozoon canis infecting an Israeli dog were investigated using next-generation and standard sequencing technologies. A complete apicoplast genome and several mitochondrion-associated sequences were generated. The apicoplast genome (31,869 bp) possessed two copies of both large subunit (23S) and small subunit (16S) ribosomal RNA genes (rDNA) within an inverted repeat region, as well as 22 protein-coding sequences, 25 transfer RNA genes (tDNA) and seven open reading frames of unknown function. Although circular-mapping, the apicoplast genome was physically linear according to next-generation data. Unlike other apicoplast genomes, genes encoding ribosomal protein S19 and tDNAs for alanine, aspartic acid, histidine, threonine and valine were not identified. No complete mitochondrial genome was recovered using next-generation data or directed PCR amplifications. Eight mitochondrion-associated (215-3523 bp) contigs assembled from next-generation data encoded a complete cytochrome c oxidase subunit I coding sequence, a complete cytochrome c oxidase subunit III coding sequence, two complete cytochrome B coding sequences, a non-coding, pseudogene for cytochrome B and multiple fragm...Continue Reading

Citations

Sep 5, 2019·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Lívia PerlesMarcos Rogério André
Jul 30, 2020·Parasitology Research·L PacificoV Veneziano
Jul 14, 2019·Scientific Reports·L PerlesM R André
Aug 23, 2020·Parasitology Research·Daniel de Abreu Reis FerreiraMarcos Rogério André
Nov 13, 2020·Parasites & Vectors·D James Harris
Dec 15, 2020·Journal of the South African Veterinary Association·David J HarrisFrancois Roux
Apr 27, 2021·International Journal for Parasitology. Parasites and Wildlife·Edward C NetherlandsBarend L Penzhorn
Aug 18, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Germán A Gutierrez-LiberatoNubia E Matta

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