Abstract
Cancer genome profiling of cytology specimens using next-generation sequencing (NGS) requires adequate and good-quality DNA. Genomic examination of cytology samples was conventionally performed on cell block (CB) or smear specimens than on residual liquid-based cytology (LBC) specimens, which are high-quality DNA sources even after long-term storage. We estimated tumor fractions of 37 residual LBC specimens, including 30 fine needle aspiration (FNA) samples from the thyroid (12 papillary thyroid carcinomas and two malignant lymphomas), lymph node (13 metastatic carcinomas and one malignant lymphoma), and breast cancer (one phyllodes tumor and one invasive ductal carcinoma), two pancreatic carcinoma samples, and five liquid (ascites, pleural effusion, and cerebrospinal fluid) samples. The DNA was extracted from all samples and subjected to NGS using a customized cancer gene panel comprising 28 cancer-related genes. NGS analysis revealed somatic mutations corresponding to pathological diagnosis with adequate variant allele frequency (VAF) in 24 LBC specimens, which had significantly higher tumor fraction (72.5% ± 4.9%). Ten cases, including the five fluid samples, had very small tumor fractions (7.5% ± 2.3%) to obtain sufficient ...Continue Reading
References
Jul 14, 2010·The Lancet Oncology·Wendy De RoockSabine Tejpar
Dec 7, 2011·Diagnostic Cytopathology·Stewart M KnoeppMichael H Roh
Feb 7, 2015·Cancer Cytopathology·Ronak H ShahMichael F Berger
May 9, 2015·Cancer Cytopathology·Shuko HaradaIsam A Eltoum
May 15, 2015·American Journal of Clinical Pathology·Ferga C GleesonMichael J Levy
Jun 13, 2015·Scientific Reports·Tian QiuZhihui Zhang
Aug 1, 2015·Cancer Cytopathology·Sinchita Roy-ChowdhuriJohn Stewart
Dec 20, 2015·Cancer Cytopathology·Shuanzeng WeiCindy McGrath
Apr 14, 2016·The Journal of Pathology·Zheming LuBaoguo Liu
Jun 23, 2016·Archives of Pathology & Laboratory Medicine·Sinchita Roy-Chowdhuri, John Stewart
Sep 3, 2016·Archives of Pathology & Laboratory Medicine·Shaozhou Ken TianMaria E Arcila
Nov 30, 2016·Oncology Letters·Eri KametaShin Maeda
Jan 14, 2017·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Sinchita Roy-ChowdhuriRajyalakshmi Luthra
Jan 14, 2017·Cancer Cytopathology·Jordan P ReynoldsYu-Wei Cheng
Jun 6, 2017·Nature Communications·Lei YeWeiqing Wang
Jul 21, 2017·Cancer Cytopathology·David H HwangLynette M Sholl
Jul 26, 2017·Journal of Clinical Pathology·Claudio BellevicineGiancarlo Troncone
Feb 13, 2018·Pathology International·Yae KanaiYoshinao Oda
Feb 22, 2018·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Sinchita Roy-ChowdhuriRajyalakshmi Luthra
Mar 17, 2018·Archives of Pathology & Laboratory Medicine·Deepali Jain, Sinchita Roy-Chowdhuri
Apr 7, 2018·Cell·Francisco Sanchez-VegaNikolaus Schultz
Jan 9, 2019·Cancer Cytopathology·Wenrui YeSinchita Roy-Chowdhuri
Mar 17, 2019·Virchows Archiv : an International Journal of Pathology·Sinchita Roy-ChowdhuriFernando Schmitt
Mar 28, 2019·International Journal of Endocrinology·Feng-Xia YuNing Lyu
Jun 15, 2019·PloS One·Toshiaki AkahaneAkihide Tanimoto
Jan 31, 2020·Thyroid : Official Journal of the American Thyroid Association·Haixia GuanStephanie L Lee
Feb 8, 2020·Diagnostic Cytopathology·Hatice SeneldirOrhan Alimoglu
Feb 9, 2020·Diagnostics·Takeshi NishikawaChiho Ohbayashi
Mar 4, 2020·Diagnostic Cytopathology·Ryota TanakaHaruhiko Kondo
Apr 1, 2020·Diagnostic Cytopathology·Daniel Pinto, Fernando Schmitt
Citations
Nov 12, 2020·Cancers·Dana M HartlSophie Leboulleux
Jun 3, 2021·Cancer Cytopathology·Marco Dell'AquilaEsther Diana Rossi
Jul 29, 2021·Diagnostic Cytopathology·Toshiaki AkahaneAkihide Tanimoto
Aug 3, 2021·Diagnostic Cytopathology·Marissa TafoyaCory J Broehm
Jan 6, 2022·Diagnostic Cytopathology·Kana SugiyamaTomoyuki Yokose