Next-Generation Sequencing of Antibody Display Repertoires

Frontiers in Immunology
Romain RouetDaniel Christ

Abstract

In vitro selection technology has transformed the development of therapeutic monoclonal antibodies. Using methods such as phage, ribosome, and yeast display, high affinity binders can be selected from diverse repertoires. Here, we review strategies for the next-generation sequencing (NGS) of phage- and other antibody-display libraries, as well as NGS platforms and analysis tools. Moreover, we discuss recent examples relating to the use of NGS to assess library diversity, clonal enrichment, and affinity maturation.

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Sep 11, 2019·Protein Engineering, Design & Selection : PEDS·Andrew M WollacottVijaya B Kolachalama
Apr 12, 2020·Cancers·Fabio NicoliniMassimiliano Mazza
Oct 5, 2019·Scientific Reports·Nancy ChaayaSéverine Padiolleau-Lefèvre
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Apr 18, 2019·International Journal of Molecular Sciences·Chia Chiu LimTheam Soon Lim
Mar 25, 2021·Biological Chemistry·Bernhard ValldorfStefan Zielonka
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May 16, 2021·Protein Engineering, Design & Selection : PEDS·Kasandra Bélanger, Jamshid Tanha
Jul 6, 2021·Biotechnology and Bioprocess Engineering : BBE·Seung Hyun Kang, Chang-Han Lee

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Methods Mentioned

BETA
antibody display
ELISA
phage display
PCR
454 sequencing
flow cytometry
antibody-display

Software Mentioned

MorphoSys
IMPre
CD
ImmuneDB
OpenOffice
Excel
scripts
modified
High V
UCLUST

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