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Next-Generation Sequencing of Genome-Wide CRISPR Screens

Methods in Molecular Biology

Jan 1, 2018

Edwin H Yau, Tariq M Rana

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Abstract

Genome-wide functional genomic screens utilizing the clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 system have proven to be a powerful tool for systematic genomic perturbation in mammalian cells and provide an alternative to previous screens utilizing RNA inter...read more

Mentioned in this Paper

Transfection
CRISPR-Cas Systems
Shuttle Vectors
Genomic Screening
Genome
RNA
Metazoa
Cosuppression
Genome-Wide Association Study
cDNA Library
9
58
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  • Next-Generation Sequencing of Genome-Wide CRISPR Screens

    Methods in Molecular Biology

    Jan 1, 2018

    Edwin H Yau, Tariq M Rana

    PMID: 29224076

    DOI: 10.1007/978-1-4939-7514-3_13

    Abstract

    Genome-wide functional genomic screens utilizing the clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 system have proven to be a powerful tool for systematic genomic perturbation in mammalian cells and provide an alternative to previous screens utilizing RNA inter...read more

    Mentioned in this Paper

    Transfection
    CRISPR-Cas Systems
    Shuttle Vectors
    Genomic Screening
    Genome
    9
    58

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