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Next-Generation Sequencing of Genome-Wide CRISPR Screens

Methods in Molecular Biology

Dec 11, 2017

Edwin H Yau, Tariq M Rana

Abstract

Genome-wide functional genomic screens utilizing the clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 system have proven to be a powerful tool for systematic genomic perturbation in mammalian cells and provide an alternative to previous screens utilizing RNA inter...read more

Mentioned in this Paper

Genome-Wide Association Study
Shuttle Vectors
Transfection
HEK293 Cells
Genome
CRISPR-Cas Systems
Cosuppression
Genomic Screening
Laboratory
Genomics
9
88
Paper Details
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Next-Generation Sequencing of Genome-Wide CRISPR Screens

Methods in Molecular Biology

Dec 11, 2017

Edwin H Yau, Tariq M Rana

PMID: 29224076

DOI: 10.1007/978-1-4939-7514-3_13

Abstract

Genome-wide functional genomic screens utilizing the clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 system have proven to be a powerful tool for systematic genomic perturbation in mammalian cells and provide an alternative to previous screens utilizing RNA inter...read more

Mentioned in this Paper

Genome-Wide Association Study
Shuttle Vectors
Transfection
HEK293 Cells
Genome
CRISPR-Cas Systems
Cosuppression
Genomic Screening
Laboratory
Genomics
9
88

Similar Papers Found In These Feeds

CRISPR Ribonucleases Deactivation

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on mechanisms that underlie deactivation of CRISPR ribonucleases. Here is the latest research.

Genomic Techniques

Recent developments in genomics and further development of advanced post-genomic technologies, it will be possible to apply a more holistic analysis to genomic analysis. Discover the latest research in Genomic Techniques.

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