Next-generation sequencing of plasmid carrying blaOXA-48 in Klebsiella pneumoniae from Turkey

Acta Microbiologica Et Immunologica Hungarica
Azer Özad Düzgün, Ayşegül Saral

Abstract

A carbapenem-resistant Klebsiella pneumoniae strain was isolated in Turkey in 2012 and blaNDM-1 and blaOXA-48 genes were observed in this strain. The aim of this study was to investigate transferability of plasmid bearing blaOXA-48 in K. pneumoniae and to use whole-genome sequencing in order to understand the genetic context of plasmid. K. pneumoniae strain was used as donor in conjugation experiments. Antibiotic susceptibility profile of selected transconjugant was determined. Plasmid was isolated from transconjugant colony and was named as pKPT. Complete sequencing of the pKPT was conducted using a next-generation sequencing. Annotation of the contigs was performed using the Geneious R9, followed by finding open reading frames (ORFs) with selected web-based tools. BLAST analysis was performed at the NCBI BLAST server to determine genes showing more than 90% similarity with these ORFs. Results of antibiotic susceptibility test showed that transconjugant colony was resistant to ampicillin/sulbactam, piperacillin, and piperacillin/tazobactam. The pKPT plasmid had a length of 45,217 bp and an average G + C content of 49%. Blast analysis revealed that pKPT was included in the IncL/M incompatibility group. The pKPT was found to con...Continue Reading

References

Nov 1, 1978·Nucleic Acids Research·J TomassiniR J Roberts
Nov 1, 1995·Journal of Bacteriology·J G Lawrence, J R Roth
Oct 26, 2002·Journal of Bacteriology·Shinya OhtaEiichi Ohtsubo
Dec 25, 2003·Antimicrobial Agents and Chemotherapy·Laurent PoirelPatrice Nordmann
Nov 23, 2007·Proceedings of the National Academy of Sciences of the United States of America·Leslie CuthbertsonChris Whitfield
Jun 4, 2008·Antimicrobial Agents and Chemotherapy·Amélie CarrërPatrice Nordmann
Mar 25, 2009·Antimicrobial Agents and Chemotherapy·Alessandra Carattoli
Jan 21, 2010·Antimicrobial Agents and Chemotherapy·Amélie CarrërPatrice Nordmann
Jul 13, 2011·Antimicrobial Agents and Chemotherapy·Cristina PitartFrancesc Marco
Aug 10, 2011·BMC Genomics·Nabil-Fareed AlikhanScott A Beatson
Nov 16, 2011·Antimicrobial Agents and Chemotherapy·Laurent PoirelPatrice Nordmann
Apr 14, 2012·The Journal of Antimicrobial Chemotherapy·Laurent PoirelPatrice Nordmann
Apr 26, 2012·The Journal of Antimicrobial Chemotherapy·Vassiliki DimouNeil Woodford
Aug 25, 2012·Journal of Medical Microbiology·M SaïdaniI Boutiba-Ben Boubaker
Mar 19, 2013·International Journal of Medical Microbiology : IJMM·Alessandra Carattoli
Apr 12, 2013·Microbial Informatics and Experimentation·David J Edwards, Kathryn E Holt
Jun 5, 2013·Antimicrobial Agents and Chemotherapy·Sibel BergerAlain Lozniewski
Aug 10, 2013·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·A PotronP Nordmann
Oct 1, 2008·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Osman Birol OzgumusAli Osman Kilic
Apr 4, 2014·Clinical Microbiology Reviews·Benjamin A Evans, Sebastian G B Amyes
Jul 20, 2016·Antimicrobial Agents and Chemotherapy·Ding-Qiang ChenQian Wang
Jan 28, 2017·Scientific Reports·Michiru NishitaYasuhiro Minami
Mar 1, 2017·PloS One·Muhammad KamruzzamanJonathan R Iredell
May 18, 2017·Frontiers in Cellular and Infection Microbiology·Wei LiangYuan Lu
Oct 11, 2017·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Assia MairiAziz Touati

❮ Previous
Next ❯

Citations

Mar 5, 2021·Acta Microbiologica Et Immunologica Hungarica·Elvan Hortaç İştarJülide Sedef Göçmen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carbapenems (ASM)

Carbapenems are members of the beta lactam class of antibiotics and are used for the treatment of severe or high-risk bacterial infections. Discover the latest research on carbapenems here.

Carbapenems

Carbapenems are members of the beta lactam class of antibiotics and are used for the treatment of severe or high-risk bacterial infections. Discover the latest research on carbapenems here.