Next-generation sequencing reveals differentially expressed small noncoding RNAs in uterine leiomyoma

Fertility and Sterility
Tsai-Der ChuangOmid Khorram

Abstract

To determine the expression profile of small noncoding RNAs (sncRNAs) in leiomyoma, which has not been investigated to date. Laboratory-based investigation. Academic center. Women undergoing hysterectomy for benign indications. Next-generation sequencing and screening of an sncRNA database with confirmatory analysis by quantitative reverse-transcription polymerase chain reaction (qRT-PCR). Expression profile of sncRNAs in leiomyoma and matched myometrium. Screening our previously determined RNA sequencing data with the sncRNA database resulted in identification of 15 small nuclear (sn) RNAs, 284 small nucleolar (sno) RNAs, 98 Piwi-interacting (pi) RNAs, 152 transfer (t) RNAs, and 45 ribosomal (r) RNAs, of which 15 snoRNAs, 24 piRNAs, 7 tRNAs, and 6 rRNAs were differentially expressed at a 1.5-fold change cutoff in leiomyoma compared with myometrium. We selected 5 snoRNAs, 4 piRNAs, 1 tRNA, and 1 rRNA that were differentially expressed and confirmed their expression in paired tissues (n = 20) from both phases of the menstrual cycle with the use of qRT-PCR. The results indicated up-regulation of the snoRNAs (SNORD30, SNORD27, SNORA16A, SNORD46, and SNORD56) and down-regulation of the piRNAs (piR-1311, piR-16677, piR-20365, piR-41...Continue Reading

References

Jun 18, 2002·Current Opinion in Cell Biology·Witold Filipowicz, Vanda Pogacić
Dec 17, 2005·Science·Shivendra Kishore, Stefan Stamm
Jun 15, 2007·Molecular Cell·Lasse Peters, Gunter Meister
Sep 21, 2007·Nucleic Acids Research·S Sai Lakshmi, Shipra Agrawal
Nov 23, 2007·Development·Carla Klattenhoff, William Theurkauf
Dec 13, 2007·Nature Reviews. Molecular Cell Biology·Gyorgy Hutvagner, Martin J Simard
Oct 28, 2008·Seminars in Reproductive Medicine·Xiaoping Luo, Nasser Chegini
May 29, 2009·RNA·Ryan J TaftJohn S Mattick
Dec 1, 2009·Nature Structural & Molecular Biology·Bingbing WangCarl D Novina
Dec 22, 2009·Current Biology : CB·Nicolas RobineEric C Lai
Mar 17, 2010·Molecular Cell·Tamás KissBeáta E Jády
Apr 24, 2010·The FEBS Journal·Xiaoying JiangMark A Lindsay
Jul 16, 2010·Nature Reviews. Genetics·Roger P AlexanderMark B Gerstein
Jul 29, 2010·Molecular Cancer·Jipei LiaoFeng Jiang
Apr 13, 2011·Cancer Letters·Valentina MirisolaAngelo Salami
May 7, 2011·Nucleic Acids Research·Zheng YanPhilipp Khaitovich
Feb 15, 2012·Haematologica·Lucía López-CorralNorma C Gutiérrez
Mar 27, 2012·Genomics·Boryana GeorgievaVesselin Baev
Nov 28, 2012·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Marina Falaleeva, Stefan Stamm
Dec 12, 2012·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·G HuangX Zhang
Aug 21, 2013·Science·Kai Kupferschmidt
Sep 6, 2013·BMC Medical Genomics·Domenica RonchettiAntonino Neri
Jan 29, 2014·Genome Biology·Adrien Le ThomasAlexei A Aravin
Aug 28, 2014·International Journal of Cancer. Journal International Du Cancer·Lu GaoFeng Jiang
Sep 15, 2014·Molecular Cancer·Gang XuZhong-Tian Qi
Sep 16, 2014·FEBS Letters·Paul Anderson, Pavel Ivanov
Oct 9, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Da-Wei WangGui-Zhen Zhang
Dec 6, 2014·Nucleic Acids Research·Katherine E SloanMarkus T Bohnsack
Dec 18, 2014·Genome Biology·Michael I LoveSimon Anders
Feb 18, 2015·Oncotarget·Maria RavoMaurizio Guida
Apr 10, 2015·Acta Biochimica Et Biophysica Sinica·Yi TanXiangqi Li
May 27, 2015·Journal of Developmental Origins of Health and Disease·O KhorramW J Pearce
May 29, 2015·American Journal of Physiology. Cell Physiology·Tsai-Der ChuangOmid Khorram

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Citations

Aug 17, 2020·American Journal of Physiology. Heart and Circulatory Physiology·Tsai-Der ChuangVirender K Rehan
Oct 20, 2020·Fertility and Sterility·Tsai-Der ChuangOmid Khorram
Apr 13, 2021·Reproductive Sciences·Zahra FalahatiReza Mirfakhraie
Jun 13, 2021·Fertility and Sterility·Tsai-Der ChuangOmid Khorram

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