Next generation sequencing to detect variation in the Plasmodium falciparum circumsporozoite protein.

The American Journal of Tropical Medicine and Hygiene
Kavita GandhiChristopher V Plowe

Abstract

The malaria vaccine RTS,S/AS01, based on immunogenic regions of the Plasmodium falciparum circumsporozoite protein (CSP), has partial efficacy against clinical malaria in African children. Understanding how sequence diversity in CSP T- and B-cell epitopes relates to naturally acquired and vaccine-induced immunity may be useful in efforts to improve the efficacy of CSP-based vaccines. However, limitations in sequencing technology have precluded thorough evaluation of diversity in the immunogenic regions of this protein. In this study, 454, a next generation sequencing technology, was evaluated as a method for assessing diversity in these regions. Portions of the circumsporozoite gene (cs) were sequenced both by 454 and Sanger sequencing from samples collected in a study in Bandiagara, Mali. 454 detected more single nucleotide polymorphisms and haplotypes in the T-cell epitopes than Sanger sequencing, and it was better able to resolve genetic diversity in samples with multiple infections; however, it failed to generate sequence for the B-cell epitopes.

References

Aug 3, 2005·Molecular and Biochemical Parasitology·Paul HuntDavid Walliker
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Citations

Jun 12, 2013·Trends in Parasitology·Nicole MideoAndrew F Read
Apr 20, 2014·PLoS Neglected Tropical Diseases·Christian M ParobekJonathan J Juliano
Nov 27, 2015·Expert Review of Vaccines·Katie L FlanaganMagdalena Plebanski
May 20, 2015·Veterinary Parasitology·Damer P Blake
Dec 3, 2014·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Amed Ouattara, Matthew B Laurens
Nov 24, 2017·The Journal of Experimental Medicine·Stephen W ScallyJean-Philippe Julien
Aug 26, 2020·The Journal of Infectious Diseases·Sofonias K TessemaBryan Greenhouse
Apr 9, 2017·Cold Spring Harbor Perspectives in Medicine·Daniel E Neafsey, Sarah K Volkman
Oct 22, 2015·The New England Journal of Medicine·D E NeafseyD F Wirth

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