Next Generation Sequencing uncovers within-host differences in the genetic diversity of Cryptosporidium gp60 subtypes

International Journal for Parasitology
Alireza ZahediUna Ryan

Abstract

The extent of within-host genetic diversity of parasites has implications for our understanding of the epidemiology, disease severity and evolution of parasite virulence. As with many other species, our understanding of the within-host diversity of the enteric parasite Cryptosporidium is changing. The present study compared Sanger and Next Generation Sequencing of glycoprotein 60 (gp60) amplicons from Cryptosporidium hominis (n=11), Cryptosporidium parvum (n=22) and Cryptosporidium cuniculus (n=8) DNA samples from Australia and China. Sanger sequencing identified only one gp60 subtype in each DNA sample: one C. hominis subtype (IbA10G2) (n=11), four C. parvum subtypes belonging to IIa (n=3) and IId (n=19) and one C. cuniculus subtype (VbA23) (n=8). Next Generation Sequencing identified the same subtypes initially identified by Sanger sequencing, but also identified additional gp60 subtypes in C. parvum and C. cuniculus but not in C. hominis, DNA samples. The number of C. parvum and C. cuniculus subtypes identified by Next Generation Sequencing within individual DNA samples ranged from two to four, and both C. parvum IIa and IId subtype families were identified within the one host in two samples. The finding of the present study...Continue Reading

Citations

Feb 23, 2019·Parasitology Research·I HolzhausenA Daugschies
Jun 13, 2019·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Felippe Danyel Cardoso MartinsRoberta Lemos Freire
Jan 11, 2020·Frontiers in Public Health·Arthur MorrisRachel M Chalmers
Apr 25, 2019·Nature Microbiology·Jessica C Kissinger
Jan 10, 2021·International Journal for Parasitology·Muofhe Grace MphephuMaggy Ndombo Benteke Momba
Jan 18, 2021·Zoonoses and Public Health·Kamil BraimaUna Ryan
Mar 23, 2021·Food and Waterborne Parasitology·Christine A YantaRebecca A Guy
Nov 4, 2020·Veterinary Parasitology·Alireza ZahediCaroline Jacobson
Apr 14, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Kamil BraimaUna Ryan
Sep 3, 2021·Food and Waterborne Parasitology·Jennifer K O'LearyBrigid Lucey
Aug 22, 2021·The Journal of Infectious Diseases·Ines DettwilerPhilipp Olias

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