NF-κB oscillations translate into functionally related patterns of gene expression

ELife
Samuel ZambranoAlessandra Agresti

Abstract

Several transcription factors (TFs) oscillate, periodically relocating between the cytoplasm and the nucleus. NF-κB, which plays key roles in inflammation and cancer, displays oscillations whose biological advantage remains unclear. Recent work indicated that NF-κB displays sustained oscillations that can be entrained, that is, reach a persistent synchronized state through small periodic perturbations. We show here that for our GFP-p65 knock-in cells NF-κB behaves as a damped oscillator able to synchronize to a variety of periodic external perturbations with no memory. We imposed synchronous dynamics to prove that transcription of NF-κB-controlled genes also oscillates, but mature transcript levels follow three distinct patterns. Two sets of transcripts accumulate fast or slowly, respectively. Another set, comprising chemokine and chemokine receptor mRNAs, oscillates and resets at each new stimulus, with no memory of the past. We propose that TF oscillatory dynamics is a means of segmenting time to provide renewing opportunity windows for decision.

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Methods Mentioned

BETA
ubiquitination
FCS
transcription profiling
PCR
in vitro transcription

Software Mentioned

beadarray R package
Dryad
Nonlinear Equations Octave
Mfuzz R package
Bioconductor
Genome
Studio
ImageJ
ClusterProfiling R
gcc

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