NF-kappaB- and AP-1-mediated induction of human beta defensin-2 in intestinal epithelial cells by Escherichia coli Nissle 1917: a novel effect of a probiotic bacterium

Infection and Immunity
Jan WehkampEduard F Stange

Abstract

Little is known about the defensive mechanisms induced in epithelial cells by pathogenic versus probiotic bacteria. The aim of our study was to compare probiotic bacterial strains such as Escherichia coli Nissle 1917 with nonprobiotic, pathogenic and nonpathogenic bacteria with respect to innate defense mechanisms in the intestinal mucosal cell. Here we report that E. coli strain Nissle 1917 and a variety of other probiotic bacteria, including lactobacilli--in contrast to more than 40 different E. coli strains tested--strongly induce the expression of the antimicrobial peptide human beta-defensin-2 (hBD-2) in Caco-2 intestinal epithelial cells in a time- and dose-dependent manner. Induction of hBD-2 through E. coli Nissle 1917 was further confirmed by activation of the hBD-2 promoter and detection of the hBD-2 peptide in the culture supernatants of E. coli Nissle 1917-treated Caco-2 cells. Luciferase gene reporter analyses and site-directed mutagenesis experiments demonstrated that functional binding sites for NF-kappaB and AP-1 in the hBD-2 promoter are required for induction of hBD-2 through E. coli Nissle 1917. Treatment with the NF-kappaB inhibitor Helenalin, as well as with SP600125, a selective inhibitor of c-Jun N-termin...Continue Reading

References

Oct 1, 1992·Infection and Immunity·M S Donnenberg, J B Kaper
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·B L KaganR I Lehrer
Aug 1, 1989·The Journal of Clinical Investigation·R I LehrerM E Selsted
Mar 1, 1988·Biochimie·T R Klaenhammer
May 14, 1969·Journal of Molecular Biology·H W Boyer, D Roulland-Dussoix
Aug 1, 1994·Current Opinion in Immunology·T Ganz, R I Lehrer
Sep 1, 1993·FEMS Microbiology Reviews·T R Klaenhammer
Jul 1, 1995·Infection·G BlumJ Hacker
Oct 1, 1996·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·A J Whitmarsh, R J Davis
Jun 6, 2000·American Journal of Respiratory Cell and Molecular Biology·J HarderJ M Schröder
Nov 22, 2000·The Journal of Biological Chemistry·J HarderJ M Schroder
Jun 8, 2001·The American Journal of Clinical Nutrition·E Isolauri
Jul 28, 2001·European Journal of Gastroenterology & Hepatology·K Fellermann, E F Stange
Sep 13, 2001·Trends in Microbiology·G ReidB S Gan
Dec 26, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·Suttichai KrisanaprakornkitBeverly A Dale
Jun 18, 2002·Digestive Diseases and Sciences·J WehkampK Fellermann
Aug 10, 2002·European Journal of Gastroenterology & Hepatology·Jan WehkampEduard F Stange
Feb 22, 2003·Infection and Immunity·Nita H SalzmanYvonne Paterson
Apr 12, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yuko Tsutsumi-Ishii, Isao Nagaoka
Jul 4, 2003·European Journal of Gastroenterology & Hepatology·Klaus FellermannEduard F Stange
Sep 2, 2003·Nature Reviews. Immunology·Tomas Ganz

❮ Previous
Next ❯

Citations

Sep 17, 2013·EMBO Molecular Medicine·Maureen J OstaffJan Wehkamp
Feb 2, 2008·Inflammatory Bowel Diseases·Michael Schultz
Jul 16, 2008·Inflammatory Bowel Diseases·Charles VanderpoolD Brent Polk
Jul 16, 2008·Inflammatory Bowel Diseases·S C NgS C Knight
Sep 26, 2008·Inflammatory Bowel Diseases·Jan Wehkamp, Eduard F Stange
Feb 22, 2011·Inflammatory Bowel Diseases·Stina Rikke JensenSusanne Brix
Oct 14, 2008·Genes & Nutrition·N SaulnierA Gasbarrini
Dec 21, 2006·Folia Microbiologica·I Trebichavský, I Splíchal
Mar 30, 2011·Archivum Immunologiae Et Therapiae Experimentalis·Johannes StephaniJan Hendrik Niess
May 11, 2012·Applied Microbiology and Biotechnology·Pasquale BoveDaniela Fiocco
Oct 1, 2013·International Journal of Colorectal Disease·Kaija-Leena KolhoErkki Savilahti
Nov 20, 2012·Langenbeck's Archives of Surgery·Simon JägerJan Wehkamp
Aug 9, 2012·Plant Foods for Human Nutrition·Kerry L Bentley-HewittAnton Pernthaner
Sep 10, 2010·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Dennis I SonnierTimothy A Pritts
Nov 10, 2007·Current Gastroenterology Reports·Anna M Borowiec, Richard N Fedorak
May 8, 2013·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Emily Gwyer FindlayDonald J Davidson
Dec 3, 2010·Journal of Crohn's & Colitis·Jan Wehkamp, Eduard F Stange
Jun 16, 2005·Trends in Molecular Medicine·Jan Wehkamp, Eduard F Stange
Apr 25, 2006·Lancet·Daniel R GayaJack Satsangi
Nov 3, 2005·Nature Clinical Practice. Gastroenterology & Hepatology·Jan WehkampEduard F Stange
Aug 19, 2007·Nature Reviews. Microbiology·Michael R Yeaman, Nannette Y Yount
Mar 21, 2012·The Journal of Infectious Diseases·David M RodriguesMélanie G Gareau
Apr 25, 2006·Journal of Clinical Gastroenterology·Ilse J Broekaert, W Allan Walker
Oct 13, 2005·Current Opinion in Gastroenterology·Stig Bengmark
Oct 21, 2006·Current Opinion in Gastroenterology·Jan Wehkamp, Eduard F Stange
Nov 30, 2006·Current Opinion in Gastroenterology·Jan WehkampEduard F Stange
Jun 5, 2007·Current Opinion in Gastroenterology·Jan WehkampEduard F Stange
Jul 22, 2010·Nutrition Reviews·Ilja TrebichavskyAlla Splichalova
Jul 7, 2011·Nutrition Reviews·Taylor C WallaceMary Ellen Sanders
Jul 3, 2013·Antimicrobial Agents and Chemotherapy·Andreia Bergamo EstrelaWolf-Rainer Abraham
May 14, 2008·Infection and Immunity·Geraldine O Canny, Beth A McCormick

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.