NF-kappaB activation in renal tubular epithelial cells by oxalate stimulation

International Journal of Urology : Official Journal of the Japanese Urological Association
Keiichi TozawaKenjiro Kohri

Abstract

The transcription factor nuclear factor-kappaB (NF-kappaB) is involved in inflammatory and immune responses through the induction of various cytokines and growth factors. Recently, the coordinated action of NF-kappaB and activator protein-1 was reported in osteopontin (OPN) expression. In the present study, we demonstrated that oxalate induces OPN expression by activating NF-kappaB in renal tubular cells. Furthermore, we investigated the inhibitory effect of N-acetyl-L-cysteine (NAC) on NF-kappaB activation in the human renal tubular cell line. All of the experiments were carried out using human kidney-2 cells, which are human proximal tubular epithelial cells immortalized by transduction with the human papillomavirus 16E6/E7 gene. The time-dependent extraction of total protein was performed after the uptake of 0.5 mM oxalate by the cells. The NF-kappaB activation and OPN expression were examined by western blotting and immunocytochemistry. As a result of oxalate stimulation, the amount of p65 subunit in the nucleus increased significantly (P < 0.05), and NAC significantly inhibited the translocation of p65 into the nucleus (P < 0.05). These observations indicate that NAC can be used as a drug to prevent stone formation.

References

Jun 28, 1996·The Journal of Biological Chemistry·S M Kuo, P S Aronson
Mar 27, 2001·Annual Review of Pharmacology and Toxicology·D T DenhardtS R Rittling
Apr 11, 2002·Urological Research·Keiichi TozawaKenjiro Kohri
Jun 28, 2003·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Mamiko AshizawaShigeru Kohno
Jul 16, 2003·Cancer Letters·Keiichi TozawaTakashi Okamoto
Apr 22, 2004·Journal of the American Society of Nephrology : JASN·Wisam SelbiAled Phillips
Dec 31, 2004·Proceedings of the National Academy of Sciences of the United States of America·Xiaoxia ShengMichael D Ward
May 3, 2005·Urological Research·J A WessonJ G Kleinman
Aug 18, 2005·Kidney International·Hilary J VernonAmrik Sahota
Nov 4, 2006·American Journal of Physiology. Renal Physiology·James J De YoreoJohn R Hoyer
Dec 16, 2006·Journal of Investigative Medicine : the Official Publication of the American Federation for Clinical Research·Vivek KumarJohn C Lieske

❮ Previous
Next ❯

Citations

Sep 8, 2011·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Shuzo HamamotoKenjiro Kohri
Mar 29, 2019·International Journal of Urology : Official Journal of the Japanese Urological Association·Kazumi TaguchiTakahiro Yasui
Sep 7, 2019·Journal of Cellular and Molecular Medicine·Jing ShiYongji Yan
Jul 3, 2021·Oxidative Medicine and Cellular Longevity·Yue WuShaogang Wang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.