Abstract
Chronic inflammation is linked to type 2 diabetes (T2DM), so we investigated correlations between obesity, blood glucose levels, and inflammation in T2DM patients. Peripheral blood mononuclear cells (PBMCs) were collected from 40 T2DM patients (27 men, 13 women; mean age 49.63 years), and 10 non-diabetic controls (all men; mean age 38.60 years). Inflammation was measured as DNA-binding activity of nuclear factor kappaB (NF-kappaB), a key transcription factor in inflammation. Protein levels of NF-kappaB subunit p65, and NF-kappaB inhibitor IkappaBalpha were assessed by Western blot. Transcript levels for p65, IkappaBalpha, and the NF-kappaB target genes TNF-alpha, MMP-9, IL-6, IL-8, and IL-18 were measured by real-time PCR. Body mass index (BMI) and glycohemoglobin were measured for all the subjects. NF-kappaB DNA-binding activity, p65 and IkappaBalpha protein levels, and expression of IL-6, TNFalpha and MMP-9 were significantly higher in PBMCs from T2DM patients, than from non-diabetic controls. NF-kappaB binding was significantly positively associated with both BMI and homeostasis model assessment of insulin resistance (HOMA-IR). Inflammation was observed in PBMCs in T2DM patients in a Chinese population, and correlated indepe...Continue Reading
References
Jul 1, 1985·Diabetologia·D R MatthewsR C Turner
Jun 1, 1995·Diabetes Care·K W ChenW Y Fujimoto
Aug 14, 1998·Diabetes Care·M A HofmannP P Nawroth
May 21, 1999·Lancet·M I SchmidtG Heiss
Jul 24, 2001·JAMA : the Journal of the American Medical Association·A D PradhanP M Ridker
Nov 28, 2001·Diabetes·A BierhausP P Nawroth
Oct 16, 2002·Circulation·Katherine EspositoDario Giugliano
Nov 9, 2002·Science·Alexander HoffmannDavid Baltimore
Dec 20, 2002·Nature·Carl Nathan
Dec 18, 2003·The Journal of Clinical Investigation·Haiyan XuHong Chen
Feb 28, 2004·Diabetes Care·John C Pickup
Mar 6, 2004·Circulation Research·Ling LiGeneviève Renier
Jun 8, 2004·The Journal of Clinical Endocrinology and Metabolism·Erin E Kershaw, Jeffrey S Flier
Sep 15, 2004·Circulation·Husam GhanimParesh Dandona
Oct 23, 2004·Science·D E NelsonM R H White
May 14, 2005·Circulation Research·Anders H Berg, Philipp E Scherer
Jun 16, 2005·Diabetes Research and Clinical Practice·Ivan Tkác
Oct 27, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Elisabetta BugianesiGiulio Marchesini
Nov 9, 2005·Circulation·Peter W F WilsonJames B Meigs
Sep 8, 2006·Diabetes Research and Clinical Practice·Teoman DogruSezin Bingol
Dec 29, 2006·Diabetes Care·UNKNOWN American Diabetes Association
Jan 30, 2007·Diabetes Care·James B MeigsPeter W F Wilson
Apr 13, 2007·Physiological Reviews·David B SavageGerald I Shulman
Apr 25, 2007·Diabetes, Obesity & Metabolism·Kathrin Maedler
Sep 14, 2007·Scandinavian Journal of Clinical and Laboratory Investigation·O E Johansen
Nov 26, 2008·Nature Reviews. Immunology·Gökhan S Hotamisligil, Ebru Erbay
Feb 10, 2009·Current Pharmaceutical Design·J C AnsquerK Le Malicot
Citations
Feb 25, 2011·Diabetologia·M N PhamUNKNOWN Action LADA Study Group
May 3, 2013·PloS One·Jung Eun KimDae Ryong Cha
Jan 13, 2016·BMC Complementary and Alternative Medicine·Samah M Fathy, Ehab A Drees
Aug 19, 2015·Scientific Reports·Qi LiuYan Jin
Dec 12, 2012·European Journal of Endocrinology·Mariusz KuzmickiM Gorska
Apr 3, 2013·International Journal of Molecular Medicine·Xiao LiangZu-Yi Yuan
Jan 25, 2013·American Journal of Therapeutics·Angélica Jiménez-RosalesAbraham Majluf Cruz
Aug 28, 2020·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Tanima ChatterjeeMaitree Bhattacharyya
May 20, 2020·International Journal of Biological Macromolecules·Yingshu ZhaoBaojun Xu
Sep 5, 2021·Journal of Endocrinological Investigation·M I Peña-CanoR Saucedo