NF-kappaB binding activity and pro-inflammatory cytokines expression correlate with body mass index but not glycosylated hemoglobin in Chinese population

Diabetes Research and Clinical Practice
Lan HeZhiquan Liu

Abstract

Chronic inflammation is linked to type 2 diabetes (T2DM), so we investigated correlations between obesity, blood glucose levels, and inflammation in T2DM patients. Peripheral blood mononuclear cells (PBMCs) were collected from 40 T2DM patients (27 men, 13 women; mean age 49.63 years), and 10 non-diabetic controls (all men; mean age 38.60 years). Inflammation was measured as DNA-binding activity of nuclear factor kappaB (NF-kappaB), a key transcription factor in inflammation. Protein levels of NF-kappaB subunit p65, and NF-kappaB inhibitor IkappaBalpha were assessed by Western blot. Transcript levels for p65, IkappaBalpha, and the NF-kappaB target genes TNF-alpha, MMP-9, IL-6, IL-8, and IL-18 were measured by real-time PCR. Body mass index (BMI) and glycohemoglobin were measured for all the subjects. NF-kappaB DNA-binding activity, p65 and IkappaBalpha protein levels, and expression of IL-6, TNFalpha and MMP-9 were significantly higher in PBMCs from T2DM patients, than from non-diabetic controls. NF-kappaB binding was significantly positively associated with both BMI and homeostasis model assessment of insulin resistance (HOMA-IR). Inflammation was observed in PBMCs in T2DM patients in a Chinese population, and correlated indepe...Continue Reading

References

Jul 24, 2001·JAMA : the Journal of the American Medical Association·A D PradhanP M Ridker
Dec 20, 2002·Nature·Carl Nathan
Dec 18, 2003·The Journal of Clinical Investigation·Haiyan XuHong Chen
Jun 8, 2004·The Journal of Clinical Endocrinology and Metabolism·Erin E Kershaw, Jeffrey S Flier
Sep 15, 2004·Circulation·Husam GhanimParesh Dandona
May 14, 2005·Circulation Research·Anders H Berg, Philipp E Scherer
Jun 16, 2005·Diabetes Research and Clinical Practice·Ivan Tkác
Oct 27, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Elisabetta BugianesiGiulio Marchesini
Dec 29, 2006·Diabetes Care·UNKNOWN American Diabetes Association
Apr 13, 2007·Physiological Reviews·David B SavageGerald I Shulman
Apr 25, 2007·Diabetes, Obesity & Metabolism·Kathrin Maedler
Sep 14, 2007·Scandinavian Journal of Clinical and Laboratory Investigation·O E Johansen
Nov 26, 2008·Nature Reviews. Immunology·Gökhan S Hotamisligil, Ebru Erbay
Feb 10, 2009·Current Pharmaceutical Design·J C AnsquerK Le Malicot

❮ Previous
Next ❯

Citations

Apr 3, 2013·International Journal of Molecular Medicine·Xiao LiangZu-Yi Yuan
Jan 25, 2013·American Journal of Therapeutics·Angélica Jiménez-RosalesAbraham Majluf Cruz
Aug 28, 2020·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Tanima ChatterjeeMaitree Bhattacharyya
May 20, 2020·International Journal of Biological Macromolecules·Yingshu ZhaoBaojun Xu

❮ Previous
Next ❯

Related Concepts

Related Feeds

CV Disorders & Type 2 Diabetes

This feed focuses on the association of cardiovascular diseases in patients with type 2 diabetes.

Biomarkers for Type 2 Diabetes

Biomarkers can help understand chronic diseases and assist in risk prediction for prevention and early detection of diseases. Here is the latest research on biomarkers in type 2 diabetes, a disease in which the body is unable to produce or properly use insulin.