NF-kappaB2 is a putative target gene of activated Notch-1 via RBP-Jkappa.

Molecular and Cellular Biology
F OswaldR M Schmid

Abstract

NF-kappaB2 (p100/p52), a member of the NF-kappaB/Rel family of transcription factors, is involved in the regulation of a variety of genes important for immune function. Previously, we have shown that the NF-kappaB2 gene is regulated in a positive and a negative manner. Two kappaB elements within the NF-kappaB2 promoter mediate tumor necrosis factor alpha-inducible transactivation. In addition, we have shown that there exists a transcriptional repression in the absence of NF-kappaB. To identify a DNA binding activity responsible for this transcriptional repression, we have partially purified a nuclear complex, named Rep-kappaB. Here we further analyze this putative repressive binding activity. Detailed examination of Rep-kappaB-DNA interaction revealed the sequence requirements for binding to be almost identical to those of recombination signal binding protein Jkappa (RBP-Jkappa), the mammalian homolog of the protein encoded by Drosophila suppressor of hairless [Su(H)]. In addition, in electromobility shift assays, Rep-kappaB binding activity is recognized by an antibody directed against RBP-Jkappa. By performing transient-transfection assays, we show that human RBP-Jkappa represses basal as well as RelA (p65)-stimulated NF-kapp...Continue Reading

References

Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·N D PerkinsG J Nabel
Sep 28, 1995·Nature·S JarriaultA Israel
Nov 15, 1995·Genes & Development·I M VermaS Miyamoto
Jul 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·P ZagourasS Artavanis-Tsakonas
Apr 14, 1995·Science·S Artavanis-TsakonasM E Fortini
Feb 24, 1995·Cell·D Thanos, T Maniatis
Oct 21, 1994·Cell·M E Fortini, S Artavanis-Tsakonas
Jan 1, 1994·Annual Review of Immunology·P A Baeuerle, T Henkel
Aug 2, 1994·Proceedings of the National Academy of Sciences of the United States of America·S R GrossmanE Kieff
Mar 25, 1994·Nucleic Acids Research·T TunM Kawaichi
Jan 1, 1993·International Review of Cytology·M GrilliM J Lenardo
Jan 1, 1996·Annual Review of Immunology·A S Baldwin
Oct 4, 1996·Cell·P A Baeuerle, D Baltimore
Nov 1, 1996·Science·D J Van AntwerpI M Verma
Nov 12, 1996·Proceedings of the National Academy of Sciences of the United States of America·L A MilnerD I Martin

❮ Previous
Next ❯

Citations

Oct 21, 1999·Journal of Cellular Physiology·L Miele, B Osborne
Sep 1, 2000·The Journal of Biological Chemistry·T MorimuraD Kitamura
May 11, 2000·Molecular and Cellular Biology·S Jeffries, A J Capobianco
Aug 4, 2001·Molecular and Cellular Biology·T IsoY Hamamori
Oct 10, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·P ChengD I Gabrilovich
Feb 2, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shin KawamataCatherine Lavau
Oct 18, 2001·Molecular and Cellular Biology·F OswaldR M Schmid
Jan 1, 2004·The Journal of Biological Chemistry·Farrell MacKenzieAly Karsan
Mar 29, 2005·The Journal of Biological Chemistry·Li PanKenneth W Gross
Nov 15, 2005·International Journal of Cancer. Journal International Du Cancer·Zhiwei WangFazlul H Sarkar
Mar 12, 2008·The Journal of Biological Chemistry·Yeddula Narayana, Kithiganahalli Narayanaswamy Balaji
Jan 24, 2009·Nature Reviews. Immunology·Derk AmsenRichard A Flavell
Mar 18, 2009·PloS One·Kushagra BansalKithiganahalli Narayanaswamy Balaji
Mar 17, 2010·The Journal of Biological Chemistry·Baofeng JinLizi Wu
Jan 21, 2015·Frontiers in Oncology·Marcin Teodorczyk, Mirko H H Schmidt
Jul 24, 2001·Arthritis and Rheumatism·M NakazawaT Nakajima
Jan 28, 2003·Journal of Cellular Physiology·Tatsuya IsoYasuo Hamamori
Oct 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sanne WeijzenW Martin Kast
Oct 23, 2003·Nature Reviews. Cancer·Freddy Radtke, Kenneth Raj
Dec 9, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shin-Ichi Tsukumo, Koji Yasutomo
Dec 2, 2005·The EMBO Journal·Hyun Mu ShinBarbara A Osborne
Nov 18, 2005·Blood·Kevin G Leong, Aly Karsan
Dec 19, 2006·Nature Medicine·Tomas VilimasIannis Aifantis
Dec 9, 2008·Circulation Research·David MorrowPaul A Cahill
Dec 16, 2006·Nature Reviews. Immunology·Barbara A Osborne, Lisa M Minter
Nov 21, 2008·Journal of Cellular Biochemistry·Olga A MamaevaJay M McDonald
Nov 17, 2007·The EMBO Journal·Mudit Tyagi, Jonathan Karn

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Fate Specification

Cell fate specification is determined by complex signal transduction pathways including Notch and the Sonic hedgehog signaling pathway. Here is the latest research on regulation of cell fate determination.

© 2022 Meta ULC. All rights reserved