NFAT-1, Sp-1, Sp-3, and miR-21: New regulators of chemokine C receptor 7 expression in mature human dendritic cells

Human Immunology
Carine Al AkoumBassam Badran

Abstract

The chemokine C receptor 7 (CCR7) is a G-protein-coupled heptahelical receptor (GPCR) that is expressed on a wide variety of cells including memory T cells, B cells, mature dendritic cells, and cancer cells. Activated by its ligands CCL19 or CCL21, CCR7 plays a major role in metastasis of cancer cells. Recent studies demonstrated the role of NF-κB and AP-1 transcription factors in addition to let-7 microRNA in CCR7 expression. Our ChIP assays further show the binding of Sp-1, Sp-3 and NFAT-1 transcription factors to their potential binding sites in the 1Kb promoter region with the later found to inhibit whilst Sp-1, and Sp-3 were found to stimulate CCR7 expression as demonstrated by transfection assays. On the other hand, in addition to the known let-7 regulation of CCR7, we found miR-21 to have a highly conserved target region in CCR7 3'UTR and to be significantly down-regulated during the course of dendritic cell maturation, allowing for high expression of CCR7.

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Jun 9, 2016·International Journal of Molecular Sciences·Shaowen WangQingguo Ruan
Sep 19, 2017·International Journal of Cancer. Journal International Du Cancer·Christine WolfDaniel Mertens
Dec 14, 2017·Scientific Reports·Svenja F B MennensKoen van den Dries
Apr 29, 2020·Cancers·Balsam Rizeq, Mohammed Imad Malki
Jan 28, 2021·RNA Biology·Guitian HeYadong Zheng

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