NFAT regulates induction of COX-2 and apoptosis of keratinocytes in response to ultraviolet radiation exposure.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Ross J FlockhartNick J Reynolds

Abstract

The nuclear factor of activated T cells (NFAT) transcription factors are regulated by calcium/calcineurin signals and play important roles in T cells, muscle, bone, and neural tissue. NFAT is expressed in the epidermis, and although recent data suggest that NFAT is involved in the skin's responses to ultraviolet radiation (UVR), the wavelengths of radiation that activate NFAT and the biological function of UV-activated NFAT remain undefined. We demonstrate that NFAT transcriptional activity is preferentially induced by UVB wavelengths in keratinocytes. The derived action spectrum for NFAT activation indicates that NFAT transcriptional activity is inversely associated with wavelength. UVR also evoked NFAT2 nuclear translocation in a parallel wavelength-dependent fashion and both transcriptional activation and nuclear translocation were inhibited by the calcineurin inhibitor cyclosporin A. UVR also evoked NFAT2 nuclear translocation in three-dimensional skin equivalents. Evidence suggests that COX-2 contributes to UV-induced carcinogenesis. Inhibiting UV-induced NFAT activation in keratinocytes, reduced COX-2 protein induction, and increased UV-induced apoptosis. COX-2 luciferase reporters lacking functional NFAT binding sites we...Continue Reading

References

May 1, 1991·The British Journal of Dermatology·P M FarrJ N Matthews
Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·R B Setlow
Dec 1, 1984·International Journal of Dermatology·T FischerB Berne
May 1, 1981·The Journal of Investigative Dermatology·J A Parrish, K F Jaenicke
Jul 1, 1993·Calcified Tissue International·K SchirrmacherD Bingmann
Mar 28, 1997·Science·C R BealsG R Crabtree
May 1, 1997·Molecular and Cellular Biology·L LyakhN R Rice
Feb 24, 1998·Clinics in Dermatology·T Horio
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·H MiyakawaH M Kwon
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·C Lopez-RodríguezA Rao
Jun 13, 2001·Oncogene·F MaciánA Rao
Aug 9, 2001·Proceedings of the National Academy of Sciences of the United States of America·M P SantiniG P Dotto
Mar 6, 2002·The Journal of Cell Biology·Valerie Horsley, Grace K Pavlath
Jun 25, 2002·Nature Cell Biology·Sebastien JauliacAlex Toker
Jul 11, 2002·American Journal of Physiology. Cell Physiology·Dharmesh R VyasFrank W Booth
Sep 17, 2002·Methods : a Companion to Methods in Enzymology·Brian L Diffey
Feb 15, 2003·Trends in Cardiovascular Medicine·David C Hill-EubanksMark T Nelson
Feb 25, 2003·The Journal of Biological Chemistry·Joel W Neal, Neil A Clipstone
Jun 25, 2003·Toxicology·M IchihashiT Horikawa
Sep 17, 2003·Genes & Development·Patrick G HoganAnjana Rao
Jan 20, 2004·Developmental Biology·Robert A Schulz, Katherine E Yutzey
Mar 3, 2004·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·André MahnsHelmut Sies
Mar 6, 2004·Cancer Metastasis Reviews·Frank A Sinicrope, Sharlene Gill
Mar 17, 2004·Toxicology and Applied Pharmacology·Diane E HeckJeffrey D Laskin
May 4, 2004·Molecular and Cellular Biology·Heidi OkamuraAnjana Rao
Jun 18, 2004·Cancer Investigation·Charles Blanke
Nov 13, 2004·The Journal of Biological Chemistry·Alessandra B BeneditoAzad Bonni
Jan 13, 2005·Proceedings of the National Academy of Sciences of the United States of America·Subramaniam JayanthiJean Lud Cadet

❮ Previous
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Citations

Oct 21, 2009·The Journal of Biological Chemistry·Buer SenJanet Rubin
Aug 24, 2012·Journal of Radiation Research·Reem YunisDavid M Rocke
Nov 26, 2009·Photochemistry and Photobiology·Nico SmitJohannes van Pelt
Feb 9, 2012·International Journal of Molecular Sciences·César López-CamarilloLaurence A Marchat
Feb 7, 2013·The Journal of Pharmacology and Experimental Therapeutics·You-Qing CaiHui-Lin Pan
Feb 13, 2014·Journal of Enzyme Inhibition and Medicinal Chemistry·Shigeo HayashiJunji Takada
Dec 31, 2008·Mutation Research·Rodrigo FrancoMihalis I Panayiotidis
Oct 26, 2012·The Journal of Investigative Dermatology·Ralph JansNick J Reynolds
Jun 2, 2015·The Journal of Investigative Dermatology·Jennifer A LatimerMark A Birch-Machin
Dec 21, 2010·The British Journal of Dermatology·D F FiorentinoU N Sundram
Dec 18, 2013·Journal of the European Academy of Dermatology and Venereology : JEADV·T GambichlerM Skrygan
Nov 3, 2009·Journal of Molecular and Cellular Cardiology·Anthony J Muslin
Mar 1, 2011·Free Radical Biology & Medicine·Ruben E A MussonNico P M Smit
Sep 3, 2009·British Journal of Cancer·R J FlockhartP E Lovat
Jan 27, 2011·Anatomy & Cell Biology·Young Sook LeeYoung Ho Lee
Jan 7, 2011·American Journal of Physiology. Cell Physiology·Matilde AliqueDiego Rodríguez-Puyol
Sep 19, 2018·International Journal of Molecular Medicine·Novi KusumaningrumJin Ho Chung
Oct 2, 2012·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Sophie C WeatherheadNicholas J Reynolds
Apr 9, 2021·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Monia ZidaneFlorent de Vathaire
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nhung Quynh DoTae-Hoo Yi

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Methods Mentioned

BETA
nuclear translocation
transfection
electrophoresis
flow cytometry
irradiating

Software Mentioned

LCS Lite
Cell Quest Pro
Excel
GraphPad Prism
Win MDI

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