NFATc4 and ATF3 negatively regulate adiponectin gene expression in 3T3-L1 adipocytes

Diabetes
Hyun Bae KimMyeong Ho Jung

Abstract

Expression of adiponectin decreases with obesity and insulin resistance. At present, the mechanisms responsible for negatively regulating adiponectin expression in adipocytes are poorly understood. In this investigation, we analyzed the effects of 5' serial deletion constructs on the murine adiponectin promoter. Here, we identified the repressor region located between -472 and -313 bp of the promoter. Removal of the putative nuclear factor of activated T-cells (NFATs) binding site increased the promoter activity, and overexpression of NFATc4 reduced the promoter activity. Treatment with the calcium ionophore A23187, an activator of NFAT, reduced mRNA as well as promoter activity. The binding of NFATc4 to the promoter was associated with increased recruitment of histone deacetylase 1 and reduced acetylation of histone H3 at the promoter site. In addition, binding of activating transcription factor 3 (ATF3) to the putative activator protein-1 site located adjacent to the NFAT binding site also repressed the promoter activity. Treatment with thapsigargin, an inducer of ATF3, reduced both mRNA and promoter activity. Importantly, the binding activities of NFATc4 and ATF3, increased significantly in white adipose tissues of ob/ob and...Continue Reading

References

Nov 1, 1996·Cell·B M Spiegelman, J S Flier
Aug 8, 1997·The Journal of Biological Chemistry·T YinJ Whelan
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·I C HoL H Glimcher
Jan 7, 1999·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·V Mohamed-AliS W Coppack
Mar 27, 1999·Biochemical and Biophysical Research Communications·Y AritaY Matsuzawa
Jan 5, 2000·The Journal of Biological Chemistry·F BlaeserT A Chatila
Apr 5, 2000·The Journal of Biological Chemistry·C D WolfgangT Hai
May 12, 2000·Journal of Structural Biology·A P Wolffe, D Guschin
Jun 10, 2000·Arteriosclerosis, Thrombosis, and Vascular Biology·K HottaY Matsuzawa
Nov 30, 2000·The Journal of Biological Chemistry·G R Crabtree
Jun 8, 2001·Annual Review of Biochemistry·S Y RothC D Allis
Jul 27, 2001·International Journal of Experimental Diabetes Research·M A StatnickJ F Caro
Sep 5, 2001·Current Opinion in Genetics & Development·I A GraefG R Crabtree
Jan 19, 2002·Biochemical and Biophysical Research Communications·Mathias FasshauerRalf Paschke
Mar 28, 2002·The Journal of Biological Chemistry·Amy E Allen-JenningsTsonwin Hai
May 15, 2002·European Journal of Pharmacology·Tsu-Shuen TsaoJoachim Fruebis
May 30, 2002·Nucleic Acids Research·Yoshinori HashimotoShigetaka Kitajima
Nov 28, 2002·Molecular Cell·Shairaz BakshSteven J Burakoff
Feb 19, 2003·Biochemical and Biophysical Research Communications·Mathias FasshauerRalf Paschke
Mar 4, 2003·European Journal of Endocrinology·Juan J Díez, Pedro Iglesias
Jul 29, 2003·Diabetes Care·Manju ChandranRobert R Henry
Sep 17, 2003·Genes & Development·Patrick G HoganAnjana Rao
Jan 16, 2004·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Koichi InoueMasatoshi Hagiwara
Jan 20, 2004·Molecular and Cellular Biology·Hao-Yuan JiangRonald C Wek
Jun 9, 2004·The Journal of Experimental Medicine·Tetiana A ZaichukOlga V Volpert
Oct 16, 2004·Science·Umut OzcanGökhan S Hotamisligil

❮ Previous
Next ❯

Citations

Sep 29, 2009·Cellular and Molecular Life Sciences : CMLS·Sreelatha GopinathJasti S Rao
Nov 16, 2006·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·F WittchenW Poller
Jun 7, 2008·Journal of Biomedical Science·Jin-Ying LuWei-Shiung Yang
Apr 15, 2008·Proceedings of the National Academy of Sciences of the United States of America·Zaheer A RanaAndres Buonanno
Feb 22, 2008·Journal of Cardiovascular Pharmacology·Ramesh NatarajanAlpha A Fowler
May 15, 2007·Current Opinion in Lipidology·Cristina Lara-CastroW Timothy Garvey
Sep 16, 2011·Clinical and Experimental Pharmacology & Physiology·Hui SuXin-Liang Ma
Aug 16, 2006·Molecular and Cellular Biology·Teddy T C YangChi-Wing Chow
Jan 5, 2012·American Journal of Physiology. Endocrinology and Metabolism·Banumathi K ColeSwarup K Chakrabarti
Jan 22, 2010·The Journal of Clinical Endocrinology and Metabolism·Gregorio ChazenbalkRicardo Azziz
Jun 19, 2009·Expert Opinion on Therapeutic Targets·Evan RosenZhao Xu
Mar 27, 2012·Diabetes Research and Clinical Practice·Lina PeiYingyan Zhang
Aug 11, 2010·Biochemical and Biophysical Research Communications·Hyun Jin ParkMyeong Ho Jung
Jul 1, 2015·Free Radical Biology & Medicine·Ramin EmraniJosiane Cillard
Apr 16, 2016·Cytokine & Growth Factor Reviews·Ganji Purnachandra NagarajuSubasini Pattnaik
Dec 17, 2009·The Biochemical Journal·Meilian Liu, Feng Liu
Aug 9, 2011·Biochemical and Biophysical Research Communications·Min Kyung JangMyeong Ho Jung
Mar 4, 2009·Cell Metabolism·Ling QiMarc Montminy
Nov 26, 2009·Biochemical and Biophysical Research Communications·Hyun Bae KimMyeong Ho Jung
Jan 22, 2013·Biochemical and Biophysical Research Communications·Min-Kyung JangMyeong Ho Jung
Feb 18, 2011·Clinica Chimica Acta; International Journal of Clinical Chemistry·Semon WuYu-Lin Ko
Dec 3, 2014·Biochemical and Biophysical Research Communications·Min-Kyung Jang, Myeong Ho Jung
Dec 3, 2014·Biochemical and Biophysical Research Communications·Min-Kyung Jang, Myeong Ho Jung
Apr 6, 2012·Biochemical and Biophysical Research Communications·Min Kyung JangMyeong Ho Jung
Apr 30, 2016·Journal of Ethnopharmacology·Yan Ping WangClara Bik San Lau
Feb 18, 2017·International Journal of Molecular Sciences·Daisuke AriyasuYukihiro Hasegawa
May 23, 2018·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Seung-Whan KimO-Yu Kwon
Sep 6, 2007·Journal of the Cardiometabolic Syndrome·Naftali SternYona Greenman
May 28, 2019·International Journal of Molecular Sciences·Sheetal ParidaDipali Sharma
Mar 22, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jung Seok HwangHan Geuk Seo
Jul 10, 2020·The Journal of Clinical Hypertension·Emilia Roy-VallejoDiego Real de Asúa
Feb 8, 2013·Endocrinology·Shu-Huei TsaiLee-Ming Chuang
Apr 10, 2013·Endocrinology·Maria SchindlerAnne Navarrete Santos
Nov 8, 2013·Molecular Endocrinology·Dagan MaoJohn S Davis
Mar 28, 2012·Hormones : International Journal of Endocrinology and Metabolism·Adeeb ShehzadYoung Sup Lee
Jan 30, 2019·Scientific Reports·Baptiste RodeDavid J Beech

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biomarkers for Type 2 Diabetes

Biomarkers can help understand chronic diseases and assist in risk prediction for prevention and early detection of diseases. Here is the latest research on biomarkers in type 2 diabetes, a disease in which the body is unable to produce or properly use insulin.

American Diabetes Association Journals

Discover the latest diabetes research published by the journals from the American Diabetes Association.