NFκB and Survivin-Mediated Radio-Adaptive Response

Radiation Research
David J GrdinaJian Jian Li

Abstract

A survivin-mediated radio-adaptive response was induced in SA-NH murine sarcoma cells following activation of nuclear transcription factor κB (NFκB) by very low doses of ionizing radiation of 5, 20 or 100 mGy. SA-NH cells and a clone stably transfected with a plasmid containing a mutated IκBα gene that prevents the activation of NFκB (SA-NH+mIκBα1) were used to investigate the role of NFκB activation in the development and expression of the survivin-mediated radio-adaptive response. Tumor cells were exposed to very low doses of radiation 30 min prior to or at times ranging from 30 min to 6 h after the first of two 2 Gy doses separated by 24 h under in vitro conditions. Evidence of very low dose radiation induced a radio-adaptive response only in SA-NH but not SA-NH+mIκBα1 cells was shown by both an increase in SA-NH cell survival of 20-40% using a standard colony forming assay and reduced apoptosis frequencies of 20-40% as determined by the TUNEL assay. Changes in survivin protein levels as a function of irradiation conditions were monitored by Western blot. A 100 mGy exposure 30 min prior to a 2 Gy dose resulted in an elevation in total survivin protein 24 h later in SA-NH but not SA-NH+mIκBα1 cells. Transfection of cells with...Continue Reading

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Citations

Nov 17, 2015·International Journal of Molecular Sciences·Byeong Mo KimYonggeun Hong
Dec 14, 2017·International Journal of Radiation Biology·Benjamin J Blyth
Jun 20, 2019·Journal of Radiological Protection : Official Journal of the Society for Radiological Protection·Edouard I Azzam
Dec 17, 2020·Cell Biology International·Poonam YadavBhavani S Shankar

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Methods Mentioned

BETA
X-ray
transfection

Software Mentioned

NIH ImageJ
SPSS
SigmaPlot

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