PMID: 8970159Dec 1, 1996Paper

NG2 proteoglycan and the actin-binding protein fascin define separate populations of actin-containing filopodia and lamellipodia during cell spreading and migration

Molecular Biology of the Cell
X H LinWilliam B Stallcup

Abstract

The transmembrane proteoglycan NG2 is able to interact both with components of the extracellular matrix and with the actin cytoskeleton. An examination of the distribution of NG2 during cell spreading suggests that NG2 can associate with two distinct types of actin-containing cytoskeletal structures, depending on the nature of the stimulus derived from the substratum. On fibronectin-coated dishes, cell surface NG2 associates exclusively with stress fibers developing within the cell. On poly-L-lysine-coated dishes, cell surface NG2 is associated with radial processes extending from the cell periphery. Spreading on fibronectin/poly-L-lysine mixtures, as well as on matrix components such as laminin, tenascin, and type VI collagen, produces cells with mosaic characteristics, i.e., NG2 is associated with both types of structures. NG2-positive radial processes are distinct from a second population of radial structures that contain fascin. NG2-positive extensions appear to be individual self-contained units (filopodia), whereas fascin is associated with actin ribs within sheets of membrane (lamellipodia). NG2- and fascin-positive structures are often localized to opposite poles of spreading cells, suggesting a possible role for the tw...Continue Reading

References

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Citations

Sep 1, 2000·Journal of Cellular Biochemistry·D S BarrittW B Stallcup
Oct 25, 2007·Brain Cell Biology·Marzenna WiranowskaPaul E Gottschall
Apr 24, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Qingyu QinXiaoning Bi
Apr 7, 2009·Journal of Physiological Anthropology·Kenichi ShibuyaTomoko Sadamoto
Jan 2, 2010·Brain Research Reviews·Jacqueline TrotterAkiko Nishiyama
Jun 29, 2012·Journal of Cellular Biochemistry·Wei-Chun WangShao-Hsuan Kao
Aug 2, 2005·Matrix Biology : Journal of the International Society for Matrix Biology·Sabrina Cattaruzza, Roberto Perris
Mar 6, 2009·Cell Adhesion & Migration·William B Stallcup, Feng-Ju Huang
Jun 14, 2016·International Journal of Experimental Pathology·Nuor S M JamilDonald M Salter
Dec 15, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Elisa TamburiniRoberto Perris
Nov 22, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jae-Kwan KimWon-Ha Lee
Feb 6, 1998·Biochimie·B Z Katz, K M Yamada
Oct 29, 2002·Cellular Signalling·Mousumi MajumdarWilliam B Stallcup

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