NG2-proteoglycan-dependent contributions of oligodendrocyte progenitors and myeloid cells to myelin damage and repair

Journal of Neuroinflammation
Karolina Kucharova, William B Stallcup

Abstract

The NG2 proteoglycan is expressed by several cell types in demyelinated lesions and has important effects on the biology of these cells. Here we determine the cell-type-specific roles of NG2 in the oligodendrocyte progenitor cell (OPC) and myeloid cell contributions to demyelination and remyelination. We have used Cre-Lox technology to dissect the cell-type-specific contributions of NG2 to myelin damage and repair. Demyelination is induced by microinjection of 1 % lysolecithin into the spinal cord white matter of control, OPC-specific NG2-null (OPC-NG2ko), and myeloid-specific NG2-null (My-NG2ko) mice. The status of OPCs, myeloid cells, axons, and myelin is assessed by light, immunofluorescence, confocal, and electron microscopy. In OPC-NG2ko mice 1 week after lysolecithin injection, the OPC mitotic index is reduced by 40 %, resulting in 25 % fewer OPCs at 1 week and a 28 % decrease in mature oligodendrocytes at 6 weeks post-injury. The initial demyelinated lesion size is not affected in OPC-NG2ko mice, but lesion repair is delayed by reduced production of oligodendrocytes. In contrast, both the initial extent of demyelination and the kinetics of lesion repair are decreased in My-NG2ko mice. Surprisingly, the OPC mitotic index ...Continue Reading

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Citations

Oct 16, 2015·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·William B StallcupFusanori Yotsumoto
Aug 24, 2016·Frontiers in Cell and Developmental Biology·Helena S DominguesJoão B Relvas
Mar 8, 2018·Physiological Reviews·Amanda Phuong TranJerry Silver
Nov 25, 2017·Cell and Tissue Research·Lei-Lei Wang, Chun-Li Zhang
Dec 15, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Elisa TamburiniRoberto Perris
Aug 7, 2017·Journal of Cellular and Molecular Medicine·Malgorzata Ziemka-NaleczJoanna Sypecka
Jun 24, 2017·Acta Neuropathologica·Marjo S van der Knaap, Marianna Bugiani
Aug 28, 2020·Acta Neuropathologica Communications·Wenhui HuangAnja Scheller
Jun 2, 2017·Acta Neuropathologica·Maja KiticAri Waisman
Sep 22, 2017·Translational Psychiatry·D A MaasG J M Martens
May 4, 2018·Neural Regeneration Research·Karolina Kucharova, William B Stallcup
Aug 10, 2017·Cancers·Weon-Kyoo You, William B Stallcup
Jan 29, 2021·World Journal of Stem Cells·Hai-Long LiuShi-Yu Feng
Mar 23, 2021·Experimental Neurology·Jonathon Chon Teng ChioMichael G Fehlings

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Methods Mentioned

BETA
light microscopy
transmission electron microscopy
confocal microscopy
dissection

Software Mentioned

Pro Plus
Image

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