NG2 proteoglycan-expressing microglia as multipotent neural progenitors in normal and pathologic brains

Glia
Akiko YokoyamaJunya Tanaka

Abstract

Rat primary microglia (MG) acquired a multipotent property to give rise to neuroectodermal cells through two-step culture in 10 and 70% serum-supplemented media for 5 days. Such multipotent MG, called promicroglioblasts (ProMGBs), formed cell aggregates, which generated cells with neuroectodermal phenotypes shortly after their transfer into serum-free medium. As revealed by immunohistochemistry, there were a few MG expressing NG2 chondroitin sulfate proteoglycan (NG2) in the neonatal rat brain. Primary culture from the neonatal brain contained NG2+ MG, which appeared to be the source of NG2+ ProMGB aggregates. The aggregates were MG marker+/NG2+/GFAP+/NCAM+/S-100beta- and had alkaline phosphatase activity. The marked accumulation of NG2+ MG was observed close to stab wounds made in the mature rat brain. The accumulated NG2+ MG in the wound gradually decreased in number, but the cells persisted up to 150 days postlesioning. In addition, GFAP immunoreactivity increased markedly around the wound. The NG2+ MG in the wounds separated with trypsin-EDTA formed NG2+ aggregates in 70% serum-supplemented medium and then transformed into cells with neuroectodermal phenotypes in serum-free medium. Although it is difficult to separate viabl...Continue Reading

References

Apr 15, 1997·Proceedings of the National Academy of Sciences of the United States of America·M A Eglitis, E Mezey
Jun 19, 1998·Brain Research. Molecular Brain Research·D ItoS Kohsaka
Oct 13, 1999·Brain Research·S Sahin KayaM Chopp
Mar 29, 2001·Microscopy Research and Technique·G AlmazánC Guaza
Oct 18, 2001·Acta Neuropathologica·S PoulyJ Antel
Oct 23, 2001·Genesis : the Journal of Genetics and Development·L ZhuoA Messing
Oct 26, 2002·BMC Immunology·Christine Servet-DelpratGuy Mouchiroud
May 20, 2003·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Tino KleinPalle Serup
May 23, 2003·Glia·Dennis A Steindler, Eric D Laywell
Jun 20, 2003·Journal of Neuroscience Research·Yasuhide KuwabaraJunya Tanaka
Nov 25, 2003·Proceedings of the National Academy of Sciences of the United States of America·James GoolsbyDavid Trisler
Dec 3, 2003·Proceedings of the National Academy of Sciences of the United States of America·Houman D HemmatiHarley I Kornblum
Oct 21, 2004·Nature Neuroscience·A Denise R GarciaMichael V Sofroniew
Feb 3, 2005·Journal of Pharmacological Sciences·Yoshihisa KitamuraShun Shimohama
Mar 26, 2005·Proceedings of the National Academy of Sciences of the United States of America·Olafur E SigurjonssonJoel C Glover
Apr 13, 2005·Neuroscience Letters·Jin NakaharaSadakazu Aiso

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Citations

Feb 23, 2012·Cell and Tissue Research·Christian W SchmeerStefan Isenmann
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Fabiana BandeiraSuzana Herculano-Houzel
Aug 16, 2011·Stem Cells and Development·Takayuki NakagomiTomohiro Matsuyama
Mar 19, 2009·Acta Histochemica Et Cytochemica·Tetsuji MoriHisao Yamada
Nov 28, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Tzong-Shiue YuSteven G Kernie
Apr 1, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Cory A BlaissSteven G Kernie
Sep 25, 2012·Experimental Cell Research·Alexander BirbrairOsvaldo Delbono
Oct 27, 2009·Biochemical and Biophysical Research Communications·Tetsuhiro NiidomeHachiro Sugimoto
Mar 25, 2009·Brain Research·Yasuharu TakamoriHisao Yamada
Oct 29, 2009·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Anna SmirkinJunya Tanaka
Mar 10, 2016·Journal of Neuroinflammation·Rika SakumaTakayuki Nakagomi
Oct 19, 2007·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Stéphane V SizonenkoJozsef Z Kiss
May 29, 2009·The European Journal of Neuroscience·Takayuki NakagomiTomohiro Matsuyama
Jan 28, 2010·The European Journal of Neuroscience·Daisuke NakayamaAkihiko Taguchi
Nov 7, 2012·Stem Cell Research·Alexander BirbrairOsvaldo Delbono
Apr 21, 2016·Auris, Nasus, Larynx·Takashi FujiwaraNaohito Hato
Oct 25, 2011·Neuroscience·J C Durham-LeeO Nesic
Feb 23, 2012·Progress in Retinal and Eye Research·Stefanie G WohlStefan Isenmann
Jun 5, 2012·European Journal of Pharmacology·M E ChoudhuryM Nomoto
Jun 10, 2009·Developmental Biology·Rachna SehgalTeri L Belecky Adams
Jun 18, 2016·Frontiers in Cellular Neuroscience·Alejandro VillarrealAlberto J Ramos
Mar 23, 2017·Stem Cells and Development·Kotaro TatebayashiTakayuki Nakagomi
Jun 15, 2007·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Hiroaki MatsumotoJunya Tanaka

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