NGF steers microglia toward a neuroprotective phenotype

Glia
Caterina RizziAntonino Cattaneo

Abstract

Microglia are the sentinels of the brain but a clear understanding of the factors that modulate their activation in physiological and pathological conditions is still lacking. Here we demonstrate that Nerve Growth Factor (NGF) acts on microglia by steering them toward a neuroprotective and anti-inflammatory phenotype. We show that microglial cells express functional NGF receptors in vitro and ex vivo. Our transcriptomic analysis reveals how, in primary microglia, NGF treatment leads to a modulation of motility, phagocytosis and degradation pathways. At the functional level, NGF induces an increase in membrane dynamics and macropinocytosis and, in vivo, it activates an outward rectifying current that appears to modulate glutamatergic neurotransmission in nearby neurons. Since microglia are supposed to be a major player in Aβ peptide clearance in the brain, we tested the effects of NGF on its phagocytosis. NGF was shown to promote TrkA-mediated engulfment of Aβ by microglia, and to enhance its degradation. Additionally, the proinflammatory activation induced by Aβ treatment is counteracted by the concomitant administration of NGF. Moreover, by acting specifically on microglia, NGF protects neurons from the Aβ-induced loss of dend...Continue Reading

References

May 1, 1990·Journal of Neuroimmunology·E BlasiF Bistoni
Sep 4, 1987·Science·R Levi-Montalcini
Nov 1, 1996·Trends in Neurosciences·R Levi-MontalciniA Leon
Feb 7, 1998·Journal of Neurochemistry·K HeeseU Otten
Aug 14, 1998·Dementia and Geriatric Cognitive Disorders·M Eriksdotter JönhagenL O Wahlund
Jun 7, 2000·Proceedings of the National Academy of Sciences of the United States of America·S CapsoniA Cattaneo
Apr 15, 2003·Nature Neuroscience·Toshihide Yamashita, Masaya Tohyama
Aug 8, 1952·Annals of the New York Academy of Sciences·R LEVI-MONTALCINI
Jul 30, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Rithwick RajagopalMoses V Chao
Apr 27, 2005·Nature Medicine·Mark H TuszynskiJames Conner
May 17, 2005·Immunity·Lynda M Stuart, R Alan B Ezekowitz
Dec 21, 2006·Nature Reviews. Neuroscience·Michelle L BlockJau-Shyong Hong
Jan 25, 2007·Nature Reviews. Molecular Cell Biology·Christian Haass, Dennis J Selkoe
Feb 23, 2007·Molecular Biology of the Cell·Amitabha MajumdarFrederick R Maxfield
Mar 16, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ganesh M ShankarBernardo L Sabatini
May 30, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Karine L RichardSerge Rivest
Oct 28, 2008·Journal of Alzheimer's Disease : JAD·Antonino CattaneoFrancesca Paoletti
Feb 13, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Henrieta ScholtzovaThomas Wisniewski
Apr 3, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Shweta MandrekarGary E Landreth
Jul 17, 2009·Neurobiology of Aging·Mara D'OnofrioAntonino Cattaneo
Sep 4, 2009·The Journal of Clinical Investigation·John D CampbellEdith M Hessel
Dec 29, 2009·Nature Neuroscience·Wei WeiRoberto Malinow
Mar 9, 2010·CNS & Neurological Disorders Drug Targets·Shweta Mandrekar-Colucci, Gary E Landreth
Mar 20, 2010·Biochemical Society Transactions·Nils BrosePaul Skehel
Jun 17, 2010·Journal of Neural Transmission·C Y Daniel Lee, Gary E Landreth
Jun 23, 2010·Proceedings of the National Academy of Sciences of the United States of America·Simona CapsoniAntonino Cattaneo
Aug 11, 2010·Archives of Neurology·Jennifer M PlaneWenbin Deng
Jul 23, 2011·Science·Rosa C PaolicelliCornelius T Gross
Jan 31, 2012·Neuron·Mohiuddin AhmadRobert C Malenka
Mar 2, 2012·Dementia and Geriatric Cognitive Disorders·Maria Eriksdotter-JönhagenLars Wahlberg
Jul 5, 2012·Cold Spring Harbor Perspectives in Medicine·Lennart Mucke, Dennis J Selkoe
Sep 1, 2012·Nature Protocols·Gerard M J BeaudoinJyothi Arikkath
Sep 4, 2012·Molecular Neurobiology·Antonino Cattaneo, Pietro Calissano
Oct 27, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Markus MüllerLiliana Minichiello
Jan 15, 2013·Neuron·Helmut KettenmannAlexei Verkhratsky

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Citations

Sep 9, 2018·Glia·Marta FumagalliClaudia Verderio
Feb 21, 2019·Neuromodulation : Journal of the International Neuromodulation Society·Víctor M López-ÁlvarezXavier Navarro
Aug 21, 2018·Frontiers in Cellular Neuroscience·Diana MatiasVivaldo Moura-Neto
Feb 26, 2019·Frontiers in Neuroscience·Sumonto MitraMaria Eriksdotter
Feb 26, 2019·Molecular Neurobiology·Kyle KossKathryn G Todd
Dec 6, 2018·Frontiers in Physiology·Pedro Sousa-VictorJoana Neves
Feb 19, 2020·Scientific Reports·Lorna GuéniotGrégory Jouvion
Aug 7, 2020·Membranes·Xiao Peng LinPaul A Gleeson
Jan 19, 2021·Neurochemistry International·Alexei VerkhratskyJunya Tanaka
Oct 30, 2020·Frontiers in Immunology·Mathias Linnerbauer, Veit Rothhammer
Mar 26, 2021·Frontiers in Immunology·Megumi Andoh, Ryuta Koyama
Sep 30, 2021·Neurochemical Research·Nuno L Soares, Helena L A Vieira

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Methods Mentioned

BETA
transgenic
PCR
Feature Extraction
ELISA
dissection
Antibody Array
FACS
PMA
PCA
flow cytometry

Software Mentioned

RStudio
Python
Feature Extraction
pClamp10
NIH
Origin
ImageJ
FlowJo
Clampex
Excel

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