Ngn3 expression during postnatal in vitro beta cell neogenesis induced by the JAK/STAT pathway

Cell Death and Differentiation
L BaeyensLuc Bouwens

Abstract

The basic helix-loop-helix protein Neurogenin3 specifies precursor cells of the endocrine pancreas during embryonic development, and is thought to be absent postnatally. We have studied Ngn3 expression during in vitro generation of beta-cells from adult rat exocrine pancreas tissue treated with epidermal growth factor and leukaemia inhibitory factor. This treatment induced a transient expression of both Ngn3 and its upstream activator hepatocyte nuclear factor 6. Inhibition of EGF and LIF signalling by pharmacological antagonists of the JAK2/STAT3 pathway, or knockdown of Ngn3 by RNA interference prevented the generation of new insulin-positive cells. This study demonstrates that in vitro growth factor stimulation can induce recapitulation of an embryonic endocrine differentiation pathway in adult dedifferentiated exocrine cells. This could prove to be important for understanding the mechanism of beta-cell regeneration and for therapeutic ex vivo neogenesis of beta cells.

References

Apr 29, 1998·Nature Biotechnology·M RojasY Z Lin
Nov 20, 1998·Current Opinion in Biotechnology·L Naldini
Sep 7, 1999·Nature·A ApelqvistH Edlund
Mar 4, 2000·Proceedings of the National Academy of Sciences of the United States of America·G GradwohlF Guillemot
Jan 9, 2001·Nature Cell Biology·C N ShenD Tosh
Jul 13, 2002·The Journal of Pathology·Susan Bonner-Weir, Arun Sharma
Oct 3, 2002·Nature Reviews. Genetics·Michael T McManus, Phillip A Sharp
Dec 20, 2002·Mechanisms of Development·Guoqiang GuDouglas A Melton
Oct 25, 2003·Molecular Endocrinology·Stuart B SmithMichael S German
Nov 15, 2003·Science·Shohta KodamaDenise L Faustman
Jun 9, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jessy LardonLuc Bouwens
Jun 25, 2004·Nucleic Acids Research·Bingbing YuanFran Lewitter
Sep 2, 2004·Proceedings of the National Academy of Sciences of the United States of America·Rosa GasaMichael German
Mar 15, 2005·Gastroenterology·Jan Nygaard JensenJan Jensen
Oct 8, 2005·Proceedings of the National Academy of Sciences of the United States of America·Kohtaro MinamiSusumu Seino

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Citations

Jun 7, 2007·Tissue Engineering·Lye T Lock, Emmanuel S Tzanakakis
Oct 18, 2008·Diabetes, Obesity & Metabolism·L Baeyens, L Bouwens
Aug 14, 2013·BMC Systems Biology·Walter de BackLutz Brusch
Dec 8, 2010·Disease Models & Mechanisms·Yoshinori ShimajiriMichael S German
Nov 10, 2010·The Review of Diabetic Studies : RDS·Isabelle Houbracken, Luc Bouwens
Aug 7, 2010·Seminars in Cell & Developmental Biology·Patrick CollombatAhmed Mansouri
Feb 12, 2009·Gastroenterology·Luc BaeyensLuc Bouwens
Dec 24, 2015·Best Practice & Research. Clinical Endocrinology & Metabolism·Willem StaelsHarry Heimberg
May 3, 2008·Molecular and Cellular Endocrinology·Neil A HanleyTimo Otonkoski
Nov 21, 2007·Trends in Endocrinology and Metabolism : TEM·Mugdha V JoglekarAnandwardhan A Hardikar
Jul 1, 2006·Stem Cells·Nathan R TreffJon S Odorico
Dec 6, 2014·Cell Death and Differentiation·M LemperL Baeyens
Aug 12, 2014·Cell Stem Cell·Rocio SanchoAxel Behrens
Aug 16, 2013·Proceedings of the National Academy of Sciences of the United States of America·Erica P CaiMinna Woo
Sep 13, 2016·Diabetes, Obesity & Metabolism·W StaelsH Heimberg
Sep 13, 2016·Diabetes, Obesity & Metabolism·C HonoréO D Madsen
Sep 24, 2016·Cell Communication and Signaling : CCS·Michael J ShamblottDustin L McGuire
Nov 14, 2016·Molecular and Cellular Endocrinology·Solomon Afelik, Meritxell Rovira
Jan 12, 2016·PloS One·Cornelis R van der TorrenBart O Roep
May 17, 2008·Biochemical Society Transactions·Bertrand DuvilliéRaphaël Scharfmann
Dec 18, 2017·Turkish Journal of Biology = Türk Biyoloji Dergisi·Ayşegül AçiksariErdal Karaöz
Dec 31, 2020·International Journal of Molecular Sciences·Lucia La SalaAntonio E Pontiroli

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