NGT1 Is Essential for N-Acetylglucosamine-Mediated Filamentous Growth Inhibition and HXK1 Functions as a Positive Regulator of Filamentous Growth in Candida tropicalis.

International Journal of Molecular Sciences
Qiuyu ZhangYunjun Yan

Abstract

Candida tropicalis is a pathogenic fungus that can cause opportunistic infections in humans. The ability of Candida species to transition between yeast and filamentous growth forms is essential to their ability to undergo environmental adaptation and to maintain virulence. In other fungal species, such as Candida albicans, N-acetylglucosamine (GlcNAc) can induce filamentous growth, whereas it suppresses such growth in C. tropicalis. In the present study, we found that knocking out the GlcNA-specific transporter gene NGT1 was sufficient to enhance C. tropicalis filamentous growth on Lee's plus GlcNAc medium. This suggests that GlcNAc uptake into C. tropicalis cells is essential to the disruption of mycelial growth. As such, we further studied how GlcNAc catabolism-related genes were able to influence C. tropicalis filamentation. We found that HXK1 overexpression drove filamentous growth on Lee's media containing glucose and GlcNAc, whereas the deletion of the same gene disrupted this filamentous growth. Interestingly, the deletion of the DAC1 or NAG1 genes impaired C. tropicalis growth on Lee's plus GlcNAc plates. Overall, these results indicate that HXK1 can serve as a positive regulator of filamentous growth, with excess GlcNA...Continue Reading

References

Sep 23, 1997·Cell·H J LoG R Fink
Oct 29, 1997·Molecular and Cellular Biology·J C Torres-Guzmán, A Domínguez
May 6, 1999·The EMBO Journal·J Pérez-MartínA D Johnson
Dec 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·M J KumarA Datta
Oct 31, 2001·Current Microbiology·F M Pérez-Campo, A Domínguez
May 17, 2002·The Journal of Hospital Infection·V Krcmery, A J Barnes
Feb 6, 2004·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Yun-Liang YangHsiu-Jung Lo
Jun 30, 2004·Trends in Microbiology·Peter SudberyJudith Berman
Oct 12, 2004·Gene·Oliver ReussJoachim Morschhäuser
Nov 24, 2005·Current Biology : CB·Torsten KlengelFritz A Mühlschlegel
Dec 29, 2006·Molecular Biology of the Cell·Francisco J Alvarez, James B Konopka
Jun 8, 2007·Microbiology and Molecular Biology Reviews : MMBR·Subhrajit BiswasAsis Datta
Jul 11, 2008·Yeast·Sreedevi N Kutty, Rosamma Philip
Jan 1, 2009·Proceedings of the National Academy of Sciences of the United States of America·Patricia L CarlisleDavid Kadosh
May 26, 2009·Nature·Geraldine ButlerChristina A Cuomo
Dec 22, 2009·Future Microbiology·Frank C Odds
Apr 24, 2010·Journal of Medical Microbiology·Rajendra J KothavadeM H Panthaki
Jul 19, 2011·Eukaryotic Cell·Delma S ThompsonDavid Kadosh
Aug 9, 2011·Comptes rendus biologies·Manuel A S SantosGabriela R Moura
Aug 17, 2011·Nature Reviews. Microbiology·Peter E Sudbery
Dec 14, 2011·Nature Reviews. Microbiology·Neil A R GowMihai G Netea
Jan 11, 2013·Virulence·François L MayerBernhard Hube
Feb 11, 2014·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·J Guinea
Aug 1, 2015·PLoS Pathogens·Shamoon Naseem, James B Konopka
Oct 16, 2015·Molecular Microbiology·Qiuyu ZhangGuanghua Huang
Jan 28, 2017·Frontiers in Microbiology·Sarah G WhaleyP David Rogers
Oct 31, 2017·Frontiers in Microbiology·Diana L Zuza-AlvesGuilherme M Chaves
Jan 26, 2018·Seminars in Cell & Developmental Biology·Rebecca A Drummond, Michail S Lionakis
Jan 23, 2019·Current Opinion in Structural Biology·Mitsutaka Ogawa, Tetsuya Okajima
Aug 24, 2015·Clinical Liver Disease·Oliver A CornelyNatalie I Jaspers

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Methods Mentioned

BETA
PCR
RNA-Seq
gene knockout
electrophoresis

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