NH2-terminal-inserted myosin II heavy chain is expressed in smooth muscle of small muscular arteries

The American Journal of Physiology
M E DiSantoS Chacko

Abstract

We demonstrate, using reverse transcriptase-polymerase chain reaction, that, whereas abdominal aorta from rabbit consists almost entirely of myosin heavy chain (MHC) mRNA with no insert at the 5'-terminal coding region, the distributing arteries (femoral and saphenous) begin to show MHC mRNA with the 21-nucleotide insert that encodes seven amino acids in the ATP-binding region located in the myosin head. The femoral/iliac artery contains > 50% inserted mRNA, whereas the more distal saphenous artery contains > 80% inserted mRNA. This insert is also present in the smooth muscle from rat tail artery but is absent in the smooth muscle from rat aorta. The actin-activated ATPase activity of myosin from the rabbit femoral/saphenous artery is 1.7-fold higher than that of the myosin from the aorta. A concomitant increase (about twofold) in the maximum shortening velocity of the saphenous artery, compared with that of the aorta, indicates that the preponderance of the inserted myosin is associated with both an increase in the actin-activated ATPase activity and a larger maximum velocity of shortening. Furthermore, analysis of the 17-kDa essential light chain from the aorta reveals near equal quantities of the 17-kDa light chain isoforms ...Continue Reading

Citations

Oct 1, 1998·The American Journal of Physiology·M E DiSantoS Chacko
Apr 30, 1999·Physiological Reviews·M J Davis, M A Hill
Nov 26, 1999·Scandinavian Journal of Urology and Nephrology. Supplementum·J A HypoliteS Chacko
Dec 19, 2000·The Journal of Physiology·J D MatthewK Takahashi
Sep 22, 2000·Microscopy Research and Technique·G J BabuM Periasamy
Aug 21, 2002·Journal of Pediatric Gastroenterology and Nutrition·Manish R PatelJosef Neu
Jul 15, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Leopold StiebellehnerKurt R Stenmark
Nov 17, 2004·Scandinavian Journal of Urology and Nephrology. Supplementum·Samuel ChackoAlan Wein
Jun 18, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Shaohua ChangMichael E Disanto
Aug 4, 2006·International Journal of Impotence Research·P T KoiM E DiSanto
Aug 26, 2010·Physiological Genomics·Steven A Fisher
Oct 12, 2013·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Rachael P Dippold, Steven A Fisher
Nov 5, 2013·American Journal of Physiology. Heart and Circulatory Physiology·John J RehoSteven A Fisher
Nov 28, 2014·American Journal of Physiology. Cell Physiology·Xiaoxu ZhengSteven A Fisher
May 19, 2000·Journal of Pediatric Gastroenterology and Nutrition·T KinouchiT Yajima
Oct 27, 1999·The Journal of Veterinary Medical Science·H SakuraiR Matsuoka
Jan 15, 1999·Acta Physiologica Scandinavica·A V SomlyoA P Somlyo
Aug 4, 1998·The American Journal of Physiology·S L WhiteM Periasamy
Apr 10, 2002·American Journal of Physiology. Cell Physiology·Jennifer J Sherwood, Thomas J Eddinger
Jun 11, 2002·American Journal of Physiology. Cell Physiology·Yongmu ZhengMichael E DiSanto
May 14, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Kayoko HosakaToshio Ohhashi
May 13, 2006·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Maureen BashaSamuel Chacko
Jul 2, 2005·Journal of Pediatric Gastroenterology and Nutrition·Andrew G HallAnthony F Philipps
Jan 20, 2012·In Vitro Cellular & Developmental Biology. Animal·Yongmu ZhengSamuel Chacko
Feb 19, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Jonathan A Parisi, Thomas J Eddinger
Feb 24, 2001·American Journal of Physiology. Cell Physiology·J A HypoliteS Chacko
Feb 15, 2001·American Journal of Physiology. Cell Physiology·R F Sánchez-OrtizS Chacko
Aug 6, 1999·Journal of Pediatric Gastroenterology and Nutrition·T KinouchiT Yajima
Jun 27, 2002·Current Urology Reports·M E DiSantoS Chacko
Jan 28, 2003·Journal of Pediatric Gastroenterology and Nutrition·Mutsumi MotouriHiroshi Kawakami
Dec 18, 2001·American Journal of Physiology. Cell Physiology·Pawel T SzymanskiRaj K Goyal
Aug 2, 2003·American Journal of Physiology. Cell Physiology·Michael E DiSantoSamuel Chacko
Aug 15, 2000·Journal of Pediatric Gastroenterology and Nutrition·B DvorakA F Philipps
Jan 25, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Deborah H Damon

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