NHE3 and NHERF are targeted to the basolateral membrane in proximal tubules of colchicine-treated rats

Kidney International
I SabolićD Brown

Abstract

Depolymerization of microtubules in proximal tubule (PT) cells of colchicine-treated rats causes disruption of vesicle recycling and redistribution of some brush-border membrane (BBM) transporters into cytoplasmic vesicles. NHE3, an isoform of the Na+/H+ exchanger in the PT cell BBM, is acutely regulated by a variety of mechanisms, including protein trafficking and interaction with the PDZ protein, NHERF. The effects of microtubule disruption by colchicine on NHE3 trafficking in PT and the potential role of NHERF in this process have not been studied. Immunofluorescence and immunogold cytochemistry were performed on cryosections of kidney tissue, and immunoblotting of BBM isolated from the renal cortex and outer stripe of control and colchicine-treated (3.2 mg/kg, IP, a single dose 12 hours before sacrifice) rats. In cells of the convoluted PT (S1/S2 segments) of control rats, NHE3 was located mainly in the BBM; subapical endosomes were weakly stained. In cells of the straight PT (S3 segment), NHE3 was present in the BBM and in lysosomes. In colchicine-treated rats, there was a marked redistribution of NHE3 from the BBM into intracellular vesicles and the basolateral plasma membrane in the S1/S2 segments. In the S3 segment, the...Continue Reading

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Citations

Jun 30, 2005·The Journal of Membrane Biology·J BiberH Murer
May 17, 2005·Pflügers Archiv : European journal of physiology·Vladiana CrljenIvan Sabolić
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Oct 9, 2015·American Journal of Physiology. Cell Physiology·Tarunmeet GujralPradeep K Dudeja
Nov 12, 2002·American Journal of Physiology. Renal Physiology·Ivan SabolicDennis Brown

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