NHERF1/EBP50 is an organizer of polarity structures and a diagnostic marker in ependymoma

Acta Neuropathologica Communications
Maria-Magdalena GeorgescuJack M Raisanen

Abstract

NHERF1/EBP50, an adaptor protein required for epithelial morphogenesis, has been implicated in the progression of various human malignancies. NHERF1-deficient mice have intestinal brush border structural defects and we report here that they also have disorganized ependymal cilia with development of non-obstructive hydrocephalus. Examination of mouse and human brain tissues revealed highest NHERF1 expression at the apical plasma membrane of ependymal cells. In ependymal tumors, NHERF1 expression was retained in polarized membrane structures, such as microlumens, rosettes and canals, where it co-localized with some of its ligands, such as moesin and PTEN. Analysis of a comprehensive panel of 113 tumors showed robust NHERF1 labeling of microlumens in 100% of ependymomas, subependymomas, and pediatric anaplastic ependymomas, and in 67% of adult anaplastic ependymomas. NHERF1 staining was present in 35% of ependymoma cases that lacked reactivity for EMA, the routine immunohistochemical marker used for ependymoma diagnosis. NHERF1 labeling of microlumens was either absent or rarely seen in other types of brain tumors analyzed, denoting NHERF1 as a reliable diagnostic marker of ependymal tumors. Anaplastic foci and a subset of adult a...Continue Reading

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Citations

Apr 29, 2016·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Aruna NambirajanAjay Garg
May 28, 2016·Acta Neuropathologica Communications·Maria-Magdalena GeorgescuJack M Raisanen
Aug 28, 2016·Neoplasia : an International Journal for Oncology Research·Maria-Magdalena GeorgescuGilbert Cote
Oct 19, 2016·Applied Immunohistochemistry & Molecular Morphology : AIMM·Aruna NambirajanChitra Sarkar
Jan 28, 2020·American Journal of Physiology. Renal Physiology·Adrienne M Bushau-Sprinkle, Eleanor D Lederer
Aug 7, 2019·Neuro-oncology Practice·David CachiaMark R Gilbert
Feb 6, 2020·Acta Neuropathologica Communications·Maria-Magdalena Georgescu, Adriana Olar
Jan 3, 2019·Brain Pathology·Marco LosaElisabeth J Rushing
Jul 19, 2020·Acta Neuropathologica Communications·M-M GeorgescuD K Burns
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Methods Mentioned

BETA
biopsy
dissection

Software Mentioned

AxioVision Rel
ImageScope
GraphPad
GraphPad Prism

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