Nickel binding and immunological properties of the C-terminal domain of the Helicobacter pylori GroES homologue (HspA)

Molecular Microbiology
I KansauA Labigne

Abstract

Helicobacter pylori synthesizes a heat-shock protein of the GroES class. The gene encoding this protein (heat-shock protein A, HspA) was recently cloned and it was shown to be unique in structure. H. pylori HspA consists of two domains: the N-terminal domain (domain A) homologous with other GroES proteins, and a C-terminal domain (domain B) corresponding to 27 additional residues resembling a metal-binding domain. Various recombinant proteins consisting of the entire HspA polypeptide, the A domain, or the B domain were produced independently as proteins fused to maltose-binding protein (MBP). Comparison of the divalent cation binding properties of the various MBP and MBP-fused proteins allowed us to conclude that HspA binds nickel ions by means of its C-terminal domain. HspA exhibited a high and specific affinity for nickel ions in comparison with its affinity for other divalent cations (copper, zinc, cobalt). Equilibrium dialysis experiments revealed that MBP-HspA binds nickel ions with an apparent dissociation constant (Kd) of 1.8 microM and a stoichiometry of 1.9 ions per molecule. The analysis of the deduced HspA amino acid sequences encoded by 35 independent clinical isolates demonstrated the existence of two molecular var...Continue Reading

Citations

Feb 1, 1997·Research in Immunology·R L Ferrero
Jul 17, 1998·Current Opinion in Chemical Biology·S W Ragsdale
Mar 6, 1999·Current Opinion in Microbiology·A Covacci, R Rappuoli
Jan 5, 2010·Metallomics : Integrated Biometal Science·Eric L CarterRobert P Hausinger
Mar 3, 2010·Proceedings of the National Academy of Sciences of the United States of America·Shujian Cun, Hongzhe Sun
Jul 19, 2006·Clinical Microbiology Reviews·Johannes G KustersErnst J Kuipers
Mar 27, 2007·Journal of Bacteriology·Susmitha SeshadriRobert J Maier
May 11, 2002·Journal of Bacteriology·Gunther SpohnVincenzo Scarlato
May 6, 2004·Journal of Bacteriology·Gunther SpohnVincenzo Scarlato
Jul 3, 2013·Medical Mycology·Duong Thu HuongDavid S Perlin
Jul 1, 2008·Current Microbiology·Hao ZengQuan Ming Zou
Jan 6, 2007·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Robert J MaierSusmitha Seshadri
Feb 13, 2007·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Clara BelzerArnoud H M van Vliet
May 16, 2007·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Ruiguang GeHongzhe Sun
Jun 20, 2008·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Yi-Bo ZengHongzhe Sun
Dec 12, 1997·Gastroenterology·H L Mobley
Feb 6, 2007·Current Opinion in Chemical Biology·Michael R Leach, Deborah B Zamble
Apr 2, 2008·Annals of the New York Academy of Sciences·Stéphane L Benoit, Robert J Maier
Feb 27, 2016·Biochemistry·Michael J Lacasse, Deborah B Zamble
Sep 26, 2006·Protein Expression and Purification·Davide RoncaratiVincenzo Scarlato
Jun 28, 2003·FEMS Microbiology Reviews·Scott B Mulrooney, Robert P Hausinger
Nov 5, 1999·Annual Review of Microbiology·Z Ge, D E Taylor
Oct 26, 2000·Protein Expression and Purification·J SvenssonB Welin
Jul 28, 2016·Diagnostic Microbiology and Infectious Disease·Guillerm Ignacio Perez-PerezNguyen Thi Hong-Hanh
Jan 30, 2004·The Journal of Biological Chemistry·Zhenzhan ChangRobert P Hausinger
Sep 27, 2006·Chemical Biology & Drug Design·Jie DongYan-Ning Xue
Feb 13, 2019·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Weronika GonciarzMagdalena Chmiela
May 6, 2010·Natural Product Reports·Harini KaluarachchiDeborah B Zamble
Feb 24, 2012·Metallomics : Integrated Biometal Science·Ruiguang GeQinglu Zhou
Sep 11, 2004·Clinical and Diagnostic Laboratory Immunology·Peter P EamranondGuillermo I Pérez-Pérez
Apr 12, 2007·The Journal of Biological Chemistry·Michael R LeachDeborah B Zamble
Apr 20, 2011·Dalton Transactions : an International Journal of Inorganic Chemistry·Magdalena Rowinska-ZyrekHenryk Kozlowski

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