PMID: 3753142Jun 1, 1986Paper

Nickel-content of urease from Bacillus pasteurii

Archives of Microbiology
S Christians, H Kaltwasser

Abstract

Urease from Bacillus pasteurii DSM 33 was purified 34-fold to a maximum specific activity of 996.5 mumol urea min-1 mg-1 at 30 degrees C. Homogeneity was demonstrated by isoelectric focussing which showed a single protein zone corresponding to a pI of about 4.6. The native enzyme was demonstrated to have a molecular mass of 230,000 and to consist of identical subunits of 65,500, as measured by SDS electrophoresis. Radioactive 63Ni-nickel co-chromatographed with urease through gel filtration, ion-exchange, and affinity chromatography. Measuring specific radioactivity, the nickel content was found to be 1.00 (+/- 0.1) g-atom Ni per mol of subunit, and 0.82 g-atom Ni per mol as measured by atomic absorption spectrometry. This indicates that 1 atom of nickel is present in each of four subunits of the enzyme.

References

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Citations

Jan 1, 1992·Archives of Microbiology·D D McCoyR P Hausinger
Mar 1, 1992·Research in Microbiology·L F Wu
Jun 28, 2003·FEMS Microbiology Reviews·Scott B Mulrooney, Robert P Hausinger
Jan 11, 1991·Microbial Pathogenesis·D J EvansD Y Graham
Jul 12, 2005·Journal of Insect Physiology·Hitoshi KurahashiYukio Sugimura
Jan 1, 1990·FEMS Microbiology Letters·G Mörsdorf, H Kaltwasser
Oct 19, 2020·Microbial Cell Factories·Zhuolin LiuXuezhi Ding
Mar 1, 1989·Microbiological Reviews·H L Mobley, R P Hausinger
Sep 1, 1995·Microbiological Reviews·H L MobleyR P Hausinger
Mar 1, 1987·Microbiological Reviews·R P Hausinger
Mar 1, 1988·Journal of Bacteriology·S E WalzR A Hull

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