PMID: 2504284Jun 13, 1989Paper

Nickel is required for the transfer of electrons from carbon monoxide to the iron-sulfur center(s) of carbon monoxide dehydrogenase from Rhodospirillum rubrum

Biochemistry
S A EnsignP W Ludden

Abstract

The role of nickel in CO oxidation and electron flow was investigated in carbon monoxide dehydrogenase from Rhodospirillum rubrum. The Fe-S centers of oxidized, nickel-containing (holo) CO dehydrogenase were completely reduced within 1 min of exposure to CO. The Fe-S centers of oxidized, nickel-deficient (apo) CO dehydrogenase were not reduced during a 35-min incubation in the presence of CO. Apo-CO dehydrogenase Fe-S centers were reduced by dithionite. The Fe-S centers of cyanide-inhibited, holo-CO dehydrogenase were not reduced in the presence of CO but were reduced by dithionite. Treatment of apo-CO dehydrogenase with cobalt(II), zinc(II), and iron(II) resulted in association of these metal ions (0.70, 1.2, and 0.86 mol of M2+/mol, respectively) with the protein but no increase in specific activity. Purified holo-CO dehydrogenase contained 1.1 mol of nickel/mol of protein and could not be further activated upon addition of NiCl2, suggesting the presence of one catalytic nickel site on the enzyme. The M2+-treated enzymes could not be further activated by addition of NiCl2 as opposed to the untreated apoenzyme, whose activity was stimulated 50-100-fold to the level of holoenzyme upon addition of NiCl2. When placed under CO, th...Continue Reading

References

Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D BonamP W Ludden
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S W RagsdaleW E Antholine
Aug 11, 1987·Biochemistry·C T Walsh, W H Orme-Johnson
Sep 30, 1982·Biochemical and Biophysical Research Communications·S W RagsdaleD V DerVartanian
Sep 15, 1983·Biochemical and Biophysical Research Communications·S W RagsdaleD V DerVartanian

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Citations

Feb 2, 2013·Journal of the American Chemical Society·Vincent C-C WangFraser A Armstrong
Jun 21, 2001·Proceedings of the National Academy of Sciences of the United States of America·J HeoP W Ludden
May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·G O TanK O Hodgson
Oct 11, 2002·Journal of Bacteriology·Marcus T WolfePaul W Ludden
Feb 22, 2014·Inorganic Chemistry·Daniel L DuBois
Feb 18, 2003·Proceedings of the National Academy of Sciences of the United States of America·Javier SeravalliStephen W Ragsdale
Jun 8, 2011·Metallomics : Integrated Biometal Science·Güneş BenderStephen W Ragsdale
Jan 1, 1991·Critical Reviews in Biochemistry and Molecular Biology·S W Ragsdale
Dec 15, 2004·Critical Reviews in Biochemistry and Molecular Biology·Stephen W Ragsdale
Aug 11, 2015·Biochimica Et Biophysica Acta·Jessica Hadj-SaïdSébastien Dementin
May 15, 1991·Archives of Biochemistry and Biophysics·C P Pace, M T Stankovich
Dec 7, 2010·Current Opinion in Chemical Biology·Yan Kung, Catherine L Drennan
Feb 16, 2018·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Mériem MerrouchSébastien Dementin
Jan 5, 1996·The Journal of Biological Chemistry·Y HeG P Roberts
Apr 1, 1995·Journal of Bacteriology·R L KerbyG P Roberts
Jun 1, 1991·Applied and Environmental Microbiology·R D FallonM Henson
May 1, 2018·Biochemistry·Sarvind Tripathi, Thomas L Poulos

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