PMID: 2504285Jun 13, 1989Paper

Nickel-specific, slow-binding inhibition of carbon monoxide dehydrogenase from Rhodospirillum rubrum by cyanide

Biochemistry
S A EnsignP W Ludden

Abstract

The inhibition of purified carbon monoxide dehydrogenase from Rhodospirillum rubrum by cyanide was investigated in both the presence and absence of CO and electron acceptor. The inhibition was a time-dependent process exhibiting pseudo-first-order kinetics under both sets of conditions. The true second-order rate constants for inhibition were 72.2 M-1 s-1 with both substrates present and 48.9 and 79.5 M-1 s-1, respectively, for the reduced and oxidized enzymes incubated with cyanide. CO partially protected the enzyme against inhibition after 25-min incubation with 100 microM KCN. Dissociation constants of 8.46 microM (KCN) and 4.70 microM (CO) were calculated for the binding of cyanide and CO to the enzyme. Cyanide inhibition was fully reversible under an atmosphere of CO after removal of unbound cyanide. N2 was unable to reverse the inhibition. The competence of nickel-deficient (apo) CO dehydrogenase to undergo activation by NiCl2 was unaffected by prior incubation with cyanide. Cyanide inhibition of holo-CO dehydrogenase was not reversed by addition of NiCl2. 14CN- remained associated with holoenzyme but not with apoenzyme through gel filtration chromatography. These findings suggest that cyanide is a slow-binding, active-si...Continue Reading

References

Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D BonamP W Ludden
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Citations

Jun 26, 2008·Archives of Microbiology·Ellen Oelgeschläger, Michael Rother
Feb 2, 2013·Journal of the American Chemical Society·Vincent C-C WangFraser A Armstrong
Jul 9, 2009·Journal of the American Chemical Society·Jae-Hun Jeoung, Holger Dobbek
Jun 21, 2001·Proceedings of the National Academy of Sciences of the United States of America·J HeoP W Ludden
May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·G O TanK O Hodgson
Jan 1, 1991·Critical Reviews in Biochemistry and Molecular Biology·S W Ragsdale
Feb 18, 2010·Inorganica Chimica Acta·Michael T MockCharles G Riordan
Aug 25, 2007·Journal of Inorganic Biochemistry·Stephen W Ragsdale
Aug 11, 2015·Biochimica Et Biophysica Acta·Jessica Hadj-SaïdSébastien Dementin
Oct 21, 2006·Biotechnology and Bioengineering·Young S DoAlan A DiSpirito
Nov 25, 2006·Biochimica Et Biophysica Acta·Steven W SingerPaul W Ludden
Dec 7, 2010·Current Opinion in Chemical Biology·Yan Kung, Catherine L Drennan
Jan 5, 1996·The Journal of Biological Chemistry·Y HeG P Roberts
Jun 8, 2011·Metallomics : Integrated Biometal Science·Güneş BenderStephen W Ragsdale
Jun 29, 2018·Frontiers in Microbiology·Florian OswaldAnke Neumann
Jun 1, 1991·Applied and Environmental Microbiology·R D FallonM Henson

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