Nickel toxicity in P. lividus embryos: Dose dependent effects and gene expression analysis

Marine Environmental Research
Rosa BonaventuraRoberta Russo

Abstract

Many industrial activities release Nickel (Ni) in the environment with harmful effects for terrestrial and marine organisms. Despite many studies on the mechanisms of Ni toxicity are available, the understanding about its toxic effects on marine organisms is more limited. We used Paracentrotus lividus as a model to analyze the effects on the stress pathways in embryos continuously exposed to different Ni doses, ranging from 0.03 to 0.5 mM. We deeply examined the altered embryonic morphologies at 24 and 48 h after Ni exposure. Some different phenotypes have been classified, showing alterations at the expenses of the dorso-ventral axis as well as the skeleton and/or the pigment cells. At the lowest dose used, Ni mainly induced a multi-spicule phenotype observed at 24 h after treatment. On the contrary, at the highest dose of Ni (0.5 mM), 90% of embryos showed no skeleton and no pigment cells. Therefore, we focused on this dose to study protein and gene expression patterns at 24 and 48 h after exposure. Among the proteins analyzed, i.e. p38MAPK, Grp78 and Mn-SOD, only p38MAPK was induced by Ni treatment. Moreover, we analyzed the mRNA profiles of a pool of genes that are involved in stress response and in development mechanisms, i...Continue Reading

Citations

Apr 3, 2019·International Journal of Immunogenetics·Marco ChiaramonteRoberta Russo
Jan 2, 2021·Journal of Environmental Management·Rosa BonaventuraAnnalisa Pinsino
Aug 25, 2021·Environmental Science and Pollution Research International·Chayma GharredTahar Gharred

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