Nickel(II)-induced nasal epithelial toxicity and oxidative mitochondrial damage

Environmental Toxicology and Pharmacology
Yoon-Jin LeeSang Han Lee

Abstract

In probing the underlying mechanisms of nickel(II)-induced cytotoxicity on nasal epithelium, we investigated the effects of nickel(II) acetate on nasal epithelial RPMI-2650 cells. Nickel(II) elicited apoptosis, as signified by pyknotic and fragmented nuclei, increased caspase-3/7 activity, and an increase in annexin V binding, hypodiploid DNA, and Bax/Bcl-2 protein ratio. Nickel(II)-induced G2/M arrest was associated with up-regulation of p21(WAF1/CIP1) expression, decrease in phosphorylation at Thr(161) of Cdc2, and down-regulation of cyclin B1. Associated with these responses, ROS generation and mitochondrial depolarization increased in a nickel(II) concentration-dependent fashion. Pretreatment with N-acetylcysteine (NAC) attenuated these changes. p53 reporter gene assay and analyses of p53, Puma, Bax, and Bcl-2 protein levels indicated that NAC inhibited nickel(II)-induced activation of p53-mediated mitochondrial apoptotic pathway. Collectively, our study provides evidences that nickel(II) may induce oxidative damage on nasal epithelium in which antioxidant NAC protects cells against nickel(II)-induced apoptosis through the prevention of oxidative stress-mediated mitochondrial damage.

References

May 1, 1977·British Journal of Industrial Medicine·R DollL G Morgan
Nov 17, 1999·Molecular Biology of the Cell·W R TaylorG R Stark
Apr 25, 2000·Experimental Cell Research·D E Wood, E W Newcomb
Jun 6, 2000·Advanced Drug Delivery Reviews·H P MerkleM C Schmidt
Jul 27, 2000·The Journal of Biological Chemistry·V A SmitsR H Medema
Aug 10, 2000·Trends in Cell Biology·S Desagher, J C Martinou
Aug 16, 2001·Journal of Biomedical Materials Research·J C WatahaP E Lockwood
May 30, 2003·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jiuhong KangYingxian Ou
Jul 6, 2004·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Jiuhong KangQing Liu
Nov 1, 2007·Chemical Research in Toxicology·Konstantin Salnikow, Anatoly Zhitkovich
Sep 8, 2009·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Annette Wengst, Stephan Reichl
Apr 15, 2010·Journal of Toxicology and Environmental Health. Part a·Chang-Yu ChenMiao-Chen Chou
Jul 17, 2012·Antioxidants & Redox Signaling·Nathalie Dehne, Bernhard Brüne
Aug 1, 2012·Biochemical Pharmacology·Fanny CaputoLina Ghibelli
Sep 25, 2012·Cellular & Molecular Biology Letters·Paweł Hikisz, Zofia M Kiliańska
Aug 15, 2013·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Ming ZengCaigao Zhong
Jan 9, 2014·In Vitro Cellular & Developmental Biology. Animal·Nikhil Baban GhateNripendranath Mandal
Mar 4, 2014·Journal of Environmental Pathology, Toxicology and Oncology : Official Organ of the International Society for Environmental Toxicology and Cancer·Samta SharmaSangeeta Shukla
Sep 16, 2014·Annales pharmaceutiques françaises·S BoulilaH El Feki
Sep 26, 2014·Environmental Science and Pollution Research International·Rim KhlifiAmel Hamza-Chaffai
Jan 27, 2015·Archives of Toxicology·Waseem Ahmad SiddiquiHaseeb Ahsan
Mar 31, 2015·Molecules and Cells·Hyun-Lim KimYang-Hee Kim

❮ Previous
Next ❯

Citations

Jun 28, 2016·International Journal of Environmental Research and Public Health·Yue WangYong-Hui Wu
Oct 21, 2016·Biochimica Et Biophysica Acta·Barbara ZambelliStefano Ciurli
Feb 8, 2019·Journal of Veterinary Research·Sylwia TerpiłowskaAndrzej Krzysztof Siwicki
Sep 25, 2019·International Journal of Molecular Sciences·Hongrui GuoLing Zhao
Nov 25, 2020·Environmental Science and Pollution Research International·Hongrui GuoLing Zhao
Nov 30, 2019·Endocrine, Metabolic & Immune Disorders Drug Targets·Thea MagroneEmilio Jirillo
Nov 28, 2019·Endocrine, Metabolic & Immune Disorders Drug Targets·Young-Ok Son
Nov 17, 2020·EFSA Journal·UNKNOWN EFSA Panel on Contaminants in the Food Chain (CONTAM)Elsa Nielsen
Feb 12, 2021·Metallomics : Integrated Biometal Science·Ruedeemars YubolphanPornpun Vivithanaporn

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis