Abstract
Metastasis negatively affects the prognosis of hepatocellular carcinoma (HCC). In the present study, niclosamide, which is known to suppress the proliferation of HCC cells, was investigated for possible suppressant effects on the migration of HCC cells. HLF and PLC/PRF/5 HCC cells were cultured in the presence of niclosamide. Cell proliferation was analyzed using the MTS assay. Cell migration was measured by performing a scratch assay. Expression levels of cyclin D1 and matrix metalloproteinase 9 (MMP9) were analyzed by performing revers transcription-quantitative polymerase chain reaction. Compared with the control treatment, treatment with 10 µm niclosamide suppressed the proliferation of the HLF and PRL/PRF/5 cells to 49.9±3.7 and 17.9±11.5% (P<0.05), respectively. Furthermore, compared with the control treatment, treatment with 1.0 µM niclosamide downregulated the expression of cyclin D1 to 52.4±4.4 and 23.9±5.4% (P<0.05) in the HLF and PRL/PRF/5 cells, respectively. In the scratch assay, treatment of the HLF cells with niclosamide (1.0 µm) decreased the distance of the scratched line from the growing edge to 4.6±1.0 mm compared with the 9.2±1.4 mm observed with the control treatment (P<0.05). Similarly, treatment of the PR...Continue Reading
References
May 1, 1995·Pathology International·M TomizawaY Kondo
Nov 1, 1994·Journal of Gastroenterology and Hepatology·F KondoK Wada
Nov 21, 2007·Cellular Signalling·Fumi Takahashi-Yanaga, Toshiyuki Sasaguri
Sep 24, 2009·Biochemistry·Minyong ChenWei Chen
Jun 21, 2011·Journal of the National Cancer Institute·Ulrike SackUlrike Stein
Feb 22, 2012·PloS One·Dan LiuYun Bai
Apr 6, 2012·PLoS Neglected Tropical Diseases·Kabemba E MwapeSarah Gabriël
Sep 1, 2010·Experimental and Therapeutic Medicine·Minoru TomizawaTakanobu Yoshida
Apr 4, 2013·Critical Reviews in Biochemistry and Molecular Biology·Jennifer VandoorenGhislain Opdenakker
Jul 10, 2013·Journal of Hepatology·Floriane PezPhilippe Merle
Jul 24, 2013·Journal of Gastroenterology and Hepatology·Hongyang Wang, Lei Chen
Jul 31, 2013·PloS One·Yu-Qiang WangJing Su
Aug 3, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Anja WielandBjörn Scheffler
Nov 26, 2013·OncoTargets and Therapy·Minoru TomizawaTakanobu Yoshida
Jan 15, 2014·PloS One·Tinghong YeLuoting Yu
Mar 1, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Hui LiTao Xiao
Mar 13, 2014·PloS One·Rong-Xin ChenJie-Feng Cui
Mar 29, 2014·Cancer Investigation·Paul ZarogoulidisKonstantinos Zarogoulidis
Apr 16, 2014·Cancer Letters·Yonghe LiDonald J Buchsbaum
Aug 19, 2014·World Journal of Gastroenterology : WJG·Parissa TabrizianMyron E Schwartz
Citations
Jul 27, 2017·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·J Y CaoX Han
May 17, 2018·Oncotarget·Atul RanjanTomoo Iwakuma
Oct 31, 2015·Oncotarget·Harish C PalFarrukh Afaq
Sep 27, 2018·Oncology Letters·Minoru TomizawaNaoki Ishige
Dec 20, 2018·Medicinal Research Reviews·N G R Dayan ElshanMichael E Jung
Feb 27, 2018·Environmental Toxicology·Ming-Hsien ChienChao-Bin Yeh