Nicotinamide decreases cytokine-induced activation of orbital fibroblasts from patients with thyroid-associated ophthalmopathy

The Journal of Clinical Endocrinology and Metabolism
Y HiromatsuK Nonaka

Abstract

We used flow cytometry to investigate the effects of nicotinamide, an inhibitor of poly (ADP ribose) synthetase, on the cell-surface expression of cytokine-induced human leukocyte antigen (HLA)-A,B,C antigen, HLA-DR antigen, intercellular adhesion molecule 1, CD44, and Fas expression in cultured orbital fibroblasts from patients with thyroid-associated ophthalmopathy. After two to seven passages, cultured orbital fibroblasts were incubated for 3 days with interferon gamma or tumor necrosis factor alpha in the presence of nicotinamide. Nicotinamide inhibited the induction of both HLA-DR and intercellular adhesion molecule 1 expression by cytokines on fibroblasts but did not interfere with induction of HLA-A,B,C, or CD44 expression. Nicotinamide also inhibited the proliferation of orbital fibroblasts, as assessed by a [3H]-thymidine incorporation assay and cell counts. Nicotinamide also enhanced the expression of the apoptosis-mediating protein Fas on fibroblasts. Our data suggest that nicotinamide inhibits cytokine-induced activation of fibroblasts and thus may decrease the autoimmune injury to the orbit in thyroid-associated ophthalmopathy.

Citations

Mar 12, 2016·Expert Review of Clinical Pharmacology·Marek Ruchała, Nadia Sawicka-Gutaj
Jun 24, 2008·Current Eye Research·Ahmet HondurBerati Hasanreisoglu
May 29, 2004·Journal of Endocrinological Investigation·C MarcocciA Pinchera
Apr 27, 2000·Endocrine Reviews·L BartalenaC Marcocci
Nov 15, 2015·The British Journal of Ophthalmology·Jwu Jin KhongDinesh Selva
May 29, 2004·Journal of Endocrinological Investigation·L BartalenaA Pinchera
Jan 1, 2021·Frontiers in Endocrinology·Giulia LanzollaMichele Marinò

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