Nicotinamide N-methyltransferase enhances the capacity of tumorigenesis associated with the promotion of cell cycle progression in human colorectal cancer cells

Archives of Biochemistry and Biophysics
Xinyou XieJun Zhang

Abstract

Nicotinamide N-methyltransferase (NNMT), an enzyme involved in the biotransformation and detoxification of many drugs and xenobiotic compounds, has been found to be overexpressed in several malignancies, including colorectal cancer. However, the biological function of NNMT and the related mechanisms in colorectal cancer have not been elucidated. In the present study, we investigated the effects of NNMT on tumorigenesis by overexpressing NNMT in the human colorectal cancer cells line SW480 which lacks constitutive NNMT expression, and downregulating NNMT expression in HT-29 cells, which exhibit high endogenous expression of NNMT. We found that NNMT significantly accelerates cell proliferation, enhances colony formation in vitro and tumorigenicity in mice; it also inhibits apoptosis, promotes cell cycle progression, increases ATP and 1-methylnicotinamide level and decreases ROS level. We also showed that 1-methylnicotinamide accelerates cell growth, inhibits apoptosis, promotes cell cycle progression, attenuates ROS production and increases ATP level. Our results indicate that NNMT enhances the capacity of tumorigenesis associated with the inhibition of cell apoptosis and the promotion of cell cycle progression in human colorecta...Continue Reading

References

Jan 31, 1990·Clinica Chimica Acta; International Journal of Clinical Chemistry·J RiniR M Weinshilboum
Sep 17, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Markus RoesslerMichael Tacke
Jan 28, 2010·Journal of Zhejiang University. Science. B·Jun ZhangChao He
Mar 8, 2011·Cell·Douglas Hanahan, Robert A Weinberg
Sep 18, 2012·Gene·Eline Hendrike VerbonJohannes Boonstra
Feb 19, 2013·Oncogene·A RufiniG Melino
Jan 9, 2014·CA: a Cancer Journal for Clinicians·Rebecca SiegelAhmedin Jemal

Citations

Feb 9, 2018·Melanoma Research·Giulia GanzettiMonica Emanuelli
Oct 2, 2019·European Journal of Clinical Investigation·Veronica PompeiMonica Emanuelli

Related Concepts

NNMT protein, human
Adenosine Triphosphate, Chromium Ammonium Salt
Cell Cycle
Energy Metabolism
Mice, Inbred BALB C
Mice, Nude
Neoplasm Proteins
Colorectal Neoplasms
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Neoplastic

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