Nicotinamide Reduces Amyloid Precursor Protein and Presenilin 1 in Brain Tissues of Amyloid Beta-Tail Vein Injected Mice

Clinical Nutrition Research
Eun Jin Kim, Soo Jin Yang

Abstract

The purpose of this study is to investigate whether nicotinic acid (NA) and nicotinamide (NAM) reduce the Alzheimer disease (AD)-related gene expression in brain tissues of amyloid beta (Aβ)-injected mice. Male Crj:CD1 (ICR) mice were divided into 6 treatment groups; 1) control, 2) Aβ control, 3) Aβ + NA 20 mg/kg/day (NA20), 4) Aβ + NA40, 5) Aβ + NAM 200 mg/kg/day (NAM200), and 6) Aβ + NAM400. After 1-week acclimation period, the mice orally received NA or NAM once a day for a total of 7 successive days. On day 7, biotinylated Aβ42 was injected into mouse tail vein. At 5 hours after the injection, blood and tissues were collected. Aβ42 injection was confirmed by Western blot analysis of Aβ42 protein in brain tissue. NAM400 pre-treatment significantly reduced the gene expression of amyloid precursor protein and presenilin 1 in brain tissues. And, NAM200 and NAM400 pre-treatments significantly increased sirtuin 1 expression in brain tissues, which is accompanied by the decreased brain expression of nuclear factor kappa B by 2 doses of NAM. Increased expression of AD-related genes was attenuated by the NAM treatment, which suggests that NAM supplementation may be a potential preventive strategy against AD-related deleterious changes.

References

Sep 28, 2013·Current Opinion in Clinical Nutrition and Metabolic Care·Yuling Chi, Anthony A Sauve
Feb 28, 2016·Lancet·Philip ScheltensWiesje Maria Van der Flier
Sep 24, 2016·Oncotarget·Xin YeYang Yang
Oct 13, 2016·Frontiers in Aging Neuroscience·Farron L McInteeJorge Ghiso
Nov 25, 2016·Journal of Cellular Biochemistry·Mauro B FrançaElis C A Eleutherio
Feb 24, 2017·Biomolecular Concepts·Marta BolósJesús Avila

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Citations

May 28, 2019·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Devin WahlDavid G Le Couteur
Nov 9, 2018·Clinical Nutrition Research·Hee Jae LeeSoo Jin Yang
Mar 1, 2019·International Journal of Molecular Sciences·Valeria GasperiMaria Valeria Catani
Aug 21, 2021·Frontiers in Cell and Developmental Biology·Xinshi WangCheng-Long Xie

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Methods Mentioned

BETA
protein assay
electrophoresis
PCR

Software Mentioned

SPSS Statistics
StepOne

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