Nicotinamide riboside regulates inflammation and mitochondrial markers in AML12 hepatocytes

Nutrition Research and Practice
Hee Jae Lee, Soo Jin Yang

Abstract

The NAD+ precursor nicotinamide riboside (NR) is a type of vitamin B3 found in cow's milk and yeast-containing food products such as beer. Recent studies suggested that NR prevents hearing loss, high-fat diet-induced obesity, Alzheimer's disease, and mitochondrial myopathy. The objective of this study was to investigate the effects of NR on inflammation and mitochondrial biogenesis in AML12 mouse hepatocytes. A subset of hepatocytes was treated with palmitic acid (PA; 250 µM) for 48 h to induce hepatocyte steatosis. The hepatocytes were treated with NR (10 µM and 10 mM) for 24 h with and without PA. The cell viability and the levels of sirtuins, inflammatory markers, and mitochondrial markers were analyzed. Cytotoxicity of NR was examined by PrestoBlue assay. Exposure to NR had no effect on cell viability or morphology. Gene expression of sirtuin 1 (Sirt1) and Sirt3 was significantly upregulated by NR in PA-treated hepatocytes. However, Sirt1 activities were increased in hepatocytes treated with low-dose NR. Hepatic pro-inflammatory markers including tumor necrosis factor-alpha and interleukin-6 were decreased in NR-treated cells. NR upregulated anti-inflammatory molecule adiponectin, and, tended to down-regulate hepatokine fet...Continue Reading

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Citations

Jun 4, 2020·International Journal of Molecular Sciences·Estefanía de GregorioMontserrat Marí
Aug 14, 2020·The Journal of Clinical Investigation·Bo ZhouRong Tian
Oct 9, 2020·Signal Transduction and Targeted Therapy·Na XieBingwen Zou
Nov 10, 2020·Pharmacology & Therapeutics·Brandon DickeyAshok K Shetty
May 2, 2021·The Journal of Physiology·Morten DallJonas T Treebak

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Methods Mentioned

BETA
PCR
enzyme-linked immunosorbent assay
ELISA
sumoylation

Software Mentioned

ImageJ
SPSS statistics

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